公开(公告)号：EP0981747A1

公开(公告)日：2000-03-01

申请号：EP98922261.7

申请日：1998-05-14

申请人： Coulter International Corp.

发明人： SIIMAN, Olavi ,  BURSHTEYN, Alexander ,  MYLVAGANAM, Ravindra ,  RAYNOR, Robert ,  ROTH, Patricia ,  SMITH, Cecilia ,  WILKINSON, Julie

IPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/569

CPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/56977 ,  Y10S436/80 ,  Y10S436/805

摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked by an aminodextran to a fluorescent phycobiliprotein or tandem dye.

公开(公告)号：EP0981747B1

公开(公告)日：2002-07-31

申请号：EP98922261.7

申请日：1998-05-14

申请人： Coulter International Corp.

发明人： SIIMAN, Olavi ,  BURSHTEYN, Alexander ,  MYLVAGANAM, Ravindra ,  RAYNOR, Robert ,  ROTH, Patricia ,  SMITH, Cecilia ,  WILKINSON, Julie

IPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/569

CPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/56977 ,  Y10S436/80 ,  Y10S436/805