公开(公告)号：EP1303751A1

公开(公告)日：2003-04-23

申请号：EP01933047.1

申请日：2001-05-04

申请人： Coulter International Corp.

发明人： DEKA, Chiranjit ,  LEE, Song, Y. ,  SHEN, Gene, G.-Y. ,  SZYDLO, Stephen ,  TSAY, Tsong-Tseh ,  GUPTA, Ravinder

IPC分类号： G01N21/76 ,  G01N33/48

CPC分类号： C09B23/02 ,  G01N1/30 ,  G01N21/47 ,  G01N21/6428 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

摘要： The dyes of the present invention are useful for many purposes that include markers or tags for detecting the presence of a molecule or compound to which they are bound. The dyes may be either red-excitable or blue-excitable. The dyes of the invention are particularly well suited for staining of nucleic acids. For example, these dyes are particularly suitable for staining of RNA in reticulocytes. In another exemplary application, these dyes are suitable for staining DNA in nucleated red blood cells. Typically, when used in staining of nucleic acids, the dyes are formulated into reagent solutions. In addition, the invention provides compositions and methods for facilitating rapid transport of dye molecules through a cell membrane. Such rapid staining requires that a sample be contacted with a dye composition of the invention in the presence of at least one surfactant and optionally, a sulfonic acid or a salt thereof.

公开(公告)号：EP1303751B1

公开(公告)日：2005-08-10

申请号：EP01933047.1

申请日：2001-05-04

申请人： Coulter International Corp.

发明人： DEKA, Chiranjit ,  LEE, Song, Y. ,  SHEN, Gene, G.-Y. ,  SZYDLO, Stephen ,  TSAY, Tsong-Tseh ,  GUPTA, Ravinder

IPC分类号： G01N21/76 ,  G01N33/48 ,  C09B23/00

CPC分类号： C09B23/02 ,  G01N1/30 ,  G01N21/47 ,  G01N21/6428 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

摘要： The dyes of the present invention are useful for many purposes that include markers or tags for detecting the presence of a molecule or compound to which they are bound. The dyes may be either red-excitable or blue-excitable. The dyes of the invention are particularly well suited for staining of nucleic acids. For example, these dyes are particularly suitable for staining of RNA in reticulocytes. In another exemplary application, these dyes are suitable for staining DNA in nucleated red blood cells. Typically, when used in staining of nucleic acids, the dyes are formulated into reagent solutions. In addition, the invention provides compositions and methods for facilitating rapid transport of dye molecules through a cell membrane. Such rapid staining requires that a sample be contacted with a dye composition of the invention in the presence of at least one surfactant and optionally, a sulfonic acid or a salt thereof.