公开(公告)号：EP1438435B1

公开(公告)日：2007-10-31

申请号：EP02797818.8

申请日：2002-09-03

申请人： DATASCOPE INVESTMENT CORP.

发明人： KADUSHIN, James M., Datascope Investment Corp. ,  GETTS, Robert C., Datascope Investment Corp. ,  ZAK, Arieh S., Datascope Investment Corp.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2549/126 ,  C12Q2525/186 ,  C12Q2525/113

摘要： Methods are provided for blocking non-specific and specific hybridization of nucleic acid samples on a microarray. The method of the present invention comprises applying a blocking reagent to the microarray, wherein the blocking reagent comprises modified nucleotide bases, preferably LNA (Locked Nucleic Acid -modified bicyclic monomeric units with a 2'-0- 4'-C methylene bridge). In further embodiments, the method includes applying a mixture including: a) a cDNA reagent obtained from mRNA of a target sample, the cDNA having a capture sequence; b) a dendrimer with a label for emitting a detectable signal and a second nucleotide sequence complementary to the capture sequence; and c) an blocking reagent containing LNA to a microarray, for producing a detectable signal from said label whereby a hybridization pattern is generated on the microarray.