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1.发明授权
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公开(公告)号：EP0297833A2
公开(公告)日：1989-01-04
申请号：EP88305875.2
申请日：1988-06-27
CPC分类号： C07K14/5255 , A61K38/00
摘要： A physiologically active polypeptide which has a lymphotoxin activity and is stable and resistant to decomposition-causing factors during purification and storage and which is substantially free of impurities. It is useful as an antitumour agent, and has the following sequence:
摘要翻译： 一种生理活性多肽，其具有淋巴毒素活性并且在纯化和储存期间是稳定的并且对分解引起的因子具有抗性并且基本上不含杂质。 作为抗肿瘤剂有用，具有以下顺序：
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3.发明公开Lymphotoxin gene, method for its production, and lymphotoxin 失效Lymphotoxin-Gen，Verfahren zu dessen Herstellung und Lymphotoxin。
公开(公告)号：EP0230781A2
公开(公告)日：1987-08-05
申请号：EP86310109.3
申请日：1986-12-23
CPC分类号： C07K14/5255
摘要： Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin A or a mixture thereof, the resulting cells are fractionated by sucrose density gradient centrifugation method to isolate a messenger RNA in l2.6S to l4.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table l as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.
摘要翻译： 将含有产生毒素的人类T细胞杂交瘤在含有佛波醇肉豆蔻酸乙酸酯，伴刀豆球蛋白A或其混合物的培养基中培养，所得细胞通过蔗糖密度梯度离心法分级，以将12.6S中的信使RNA分离至14.6S级分 ，并且从信使RNA制备含有编码具有淋巴毒素活性的多肽的部分的基因。 该基因由本文给出的表1的碱基序列表示。 使用本发明的基因，可以通过遗传工程技术获得具有本文给出的氨基酸序列（I）或（II）的新的淋巴毒素。
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4.发明公开
公开(公告)号：EP0230781A3
公开(公告)日：1988-04-27
申请号：EP86310109
申请日：1986-12-23
CPC分类号： C07K14/5255
摘要： Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin A or a mixture thereof, the resulting cells are fractionated by sucrose density gradient centrifugation method to isolate a messenger RNA in l2.6S to l4.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table l as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.
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公开(公告)号：EP0297833B1
公开(公告)日：1995-03-22
申请号：EP88305875.2
申请日：1988-06-27
CPC分类号： C07K14/5255 , A61K38/00
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公开(公告)号：EP0297833A3
公开(公告)日：1990-05-09
申请号：EP88305875.2
申请日：1988-06-27
CPC分类号： C07K14/5255 , A61K38/00
摘要： A physiologically active polypeptide which has a lymphotoxin activity and is stable and resistant to decomposition-causing factors during purification and storage and which is substantially free of impurities. It is useful as an antitumour agent, and has the following sequence:
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