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    APPARATUS AND METHOD FOR THE DETECTION OF LOCALISED FLUCTUATIONS OF IONIC CHARGE DURING A CHEMICAL REACTION BY THE USE OF ION SENSITIVE FIELD EFFECT TRANSISTORS
    2.
    发明公开
    APPARATUS AND METHOD FOR THE DETECTION OF LOCALISED FLUCTUATIONS OF IONIC CHARGE DURING A CHEMICAL REACTION BY THE USE OF ION SENSITIVE FIELD EFFECT TRANSISTORS 有权
    设备和用于检测局部电荷波动的方法，用离子敏感的场效应晶体管的辅助的化学反应过程中

    公开(公告)号：EP2211173A1

    公开(公告)日：2010-07-28

    申请号：EP10157896.1

    申请日：2002-03-11

    申请人： DNA Electronics Ltd

    发明人： TOUMAZOU, Christofer ,  PURUSHOTHAMAN, Sunil

    IPC分类号： G01N27/414 ,  C12Q1/00 ,  C12Q1/68

    CPC分类号： C12Q1/6825 ,  C12Q2565/607 ,  G01N27/4145

    摘要： Sensor and method to detect localised fluctuations of ionic charge indicating events occuring during a chemical reaction, the sensing apparatus comprising an ion sensitive field effect transistor (ISFET) arranged to generate an electrical output signal in response to localised fluctuations of ionic charge at or adjacent the surface of the transistor, and means for detecting the electrical output signal from the field effect transistor is provided with a polymerase linked layer and the chemical reaction is DNA synthesis, and the localised fluctuations of ionic charge indicate the insertion of di-deoxynucleotide triphosphates (ddNTP) and deoxynucleotide triphosphates (dNTP) by detecting a change of hydronium ions (pH value) with a sensitive Si 3 N 4 layer of the field effect transistor.
    The method is a modification to Sanger sequencing where the final stage of electrophoretic separation and subsequent detection of nucleotides is replaced by measurements of the pH by the ISFET.

    摘要翻译： 传感器和方法来检测离子电荷指示事件的化学反应过程中存在的的局部波动，所述感测装置包括离子敏感场效应晶体管（ISFET）的布置在电输出信号，以产生响应于离子电荷的局部波动处或附近的 所述晶体管的表面，以及用于检测从所述场效应晶体管的电输出信号被提供用聚合酶联层和所述化学反应是DNA合成，和离子电荷的局部波动表明二脱氧核苷酸三磷酸的插入核苷酸（ddNTP ）和脱氧核苷酸三磷酸（的dNTP）通过检测水合氢离子（pH值为）的与敏感的Si 3 N 4层的场效应晶体管的变化。 该方法是将Sanger测序，其中电泳分离和核苷酸的后续检测的最后阶段通过将pH值由ISFET测量代替的修改。