公开(公告)号：EP3102669B1

公开(公告)日：2019-06-12

申请号：EP15704282.1

申请日：2015-02-06

申请人： DSM IP Assets B.V.

发明人： VAN RIJ, Evert Tjeerd ,  SPAANS, Martine ,  HILLEBRAND, Hendrikus Bernardus Carolus

IPC分类号： C12N1/00 ,  C12R1/07 ,  C12P21/02 ,  C12N9/24 ,  C12N9/54 ,  C12N15/75 ,  C12N9/26

公开(公告)号：EP3102669A1

公开(公告)日：2016-12-14

申请号：EP15704282.1

申请日：2015-02-06

申请人： DSM IP Assets B.V.

发明人： VAN RIJ, Evert Tjeerd ,  SPAANS, Martine ,  HILLEBRAND, Hendrikus Bernardus Carolus

IPC分类号： C12N1/00 ,  C12R1/07

CPC分类号： C12N15/75 ,  C12N9/2411 ,  C12N9/2482 ,  C12N9/54 ,  C12P21/00 ,  C12P21/02 ,  C12Y302/01008 ,  C12Y302/01133 ,  C12Y304/21062 ,  C12Y304/24028

摘要： The invention provides Bacillus host cell deficient in the production of a neutral protease and of a alkaline protease which host cell comprises a nucleic acid construct, wherein the nucleic acid construct comprises a polynucleotide encoding a compound of interest or encoding a compound involved in the synthesis of a compound of interest operably linked to a promoter sequence allowing for expression of the polynucleotide in the host cell, wherein the promoter comprises a bacteriophage promoter sequence, more preferably a bacteriophage SPO1 promoter sequence. Said host cell can advantageously be used in a method of production of a compound of interest.