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1.发明公开Whole-cell catalytic system comprising a hydantoinase, a racemase and a carbamoylase 审中-公开Ganzzelliges Katalysatorsystem das eine Hydantoinase，eine Racemase and eine Carbamideaseenthält
公开(公告)号：EP2267144A3
公开(公告)日：2011-05-25
申请号：EP10188509.3
申请日：2007-12-04
申请人： DSM IP Assets B.V.
发明人： Rusnak-Müller, Monika , May, Oliver , Hermsen, Petrus Johannes , Straatman, Henricus Martinus Maria Gerardus , Skranc, Wolfgang , Boesten, Wilhelmus Hubertus Joseph , Heemskerk, Dennis , De Lange, Ben , Sarakinos, Georgios
IPC分类号： C12P13/04 , C12P13/22 , C12P41/00 , C12P17/10 , C07C229/30 , C07C229/32 , C07D233/74 , C07D233/76
CPC分类号： C12P13/04 , C07C229/30 , C07C229/32 , C07C2601/08 , C07C2601/10 , C07C2601/14 , C07C2601/16 , C07D233/74 , C07D233/78 , C12P13/22 , C12P13/222 , C12P17/10 , C12P41/00 , C12P41/009
摘要： The present invention relates to a whole cell catalytic system for the preparation of an enantiomerically enriched a-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase and hydantoin racemase are coexpressed in a recombinant micro-organism wherein the genes coding for these three enzymes are located on a single replicon.
The present invention further relates to the use of such a whole cell catalytic system according in the preparation of an enantiomerically enriched L-α-amino acid from a corresponding hydantoin.摘要翻译： 本发明涉及一种用于从相应的乙内酰脲制备对映异构体富集的α-氨基酸的全细胞催化体系，其中将乙内酰脲酶，L-氨基甲酰基酶和乙内酰脲消旋酶共表达于重组微生物中，其中编码这三种酶的基因 位于单个复制器上。 本发明还涉及根据制备相应乙内酰脲的对映异构体富集的L-±氨基酸的这种全细胞催化体系的用途。
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2.发明公开Whole-cell catalytic system comprising a hydantoinase, a racemase and a carbamoylase 审中-公开含有乙内酰脲，一消旋酶和氨甲酰全细胞催化剂体系
公开(公告)号：EP2267144A2
公开(公告)日：2010-12-29
申请号：EP10188509.3
申请日：2007-12-04
申请人： DSM IP Assets B.V.
发明人： Rusnak-Müller, Monika , May, Oliver , Hermsen, Petrus Johannes , Straatman, Henricus Martinus Maria Gerardus , Skranc, Wolfgang , Boesten, Wilhelmus Hubertus Joseph , Heemskerk, Dennis , De Lange, Ben , Sarakinos, Georgios
IPC分类号： C12P13/04 , C12P13/22 , C12P41/00 , C12P17/10 , C07C229/30 , C07C229/32 , C07D233/74 , C07D233/76
CPC分类号： C12P13/04 , C07C229/30 , C07C229/32 , C07C2601/08 , C07C2601/10 , C07C2601/14 , C07C2601/16 , C07D233/74 , C07D233/78 , C12P13/22 , C12P13/222 , C12P17/10 , C12P41/00 , C12P41/009
摘要： The present invention relates to a whole cell catalytic system for the preparation of an enantiomerically enriched a-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase and hydantoin racemase are coexpressed in a recombinant micro-organism wherein the genes coding for these three enzymes are located on a single replicon.
The present invention further relates to the use of such a whole cell catalytic system according in the preparation of an enantiomerically enriched L-α-amino acid from a corresponding hydantoin.
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