公开(公告)号：EP2194139A1

公开(公告)日：2010-06-09

申请号：EP09177092.5

申请日：2005-01-17

申请人： DSM IP Assets B.V.

发明人： Raemaekers-Franken, Petronella Catharina ,  Nossin, Petrus Martinus Matheus ,  Brandts, Paul Maria ,  Wubbolts, Marcel Gerhardus ,  Peeters, Wijnand Peter Helena ,  Ernste, Sandra ,  Wildeman De, Stefaan Marie Andre ,  Schuermann, Martin

IPC分类号： C12P13/00 ,  C12P13/02 ,  C12P7/40 ,  C12P7/44 ,  C12N1/14 ,  C12N1/20 ,  C12N9/04 ,  C12N15/11 ,  C07C229/30 ,  C07C229/06 ,  C07D223/10 ,  C08L77/00 ,  C12P7/42

CPC分类号： C12P13/005 ,  C07D223/10 ,  C12N9/001 ,  C12P13/02

摘要： The invention relates to biochemical synthesis of 6-amino caproic acid from 6-aminohex-2-enoic acid compound or from 6-amino-2-hydroxyhexanoic acid, by treatment with an enzyme having α,β-enoate reductase activity towards molecules containing an α,β-enoate group and a primary amino group.
The invention also relates to processes for obtaining suitable genetically engineered cells for being used in such biotransformation process, and to precursor fermentation of 6-amino caproic acid from intermediates leading to 6-amino caproic acid.
Finally, the invention relates to certain novel biochemically produced compounds, namely 6-aminohex-2-enoic acid, 6-aminohexanoic acid, as well as to caprolactam produced therefrom and to nylon-6 and other derivatives produced from such biochemically produced compounds or caprolactam.

摘要翻译： 本发明涉及6-氨基己-2-烯酸化合物或6-氨基-2-羟基己酸的6-氨基己酸的生物化学合成，通过用具有±，2-烯酸酯还原酶活性的酶对含有 ±，2-乙酸酯基和伯氨基。 本发明还涉及用于获得在这种生物转化过程中使用的合适的遗传工程细胞的方法，以及从导致6-氨基己酸的中间体对6-氨基己酸的前体发酵。 最后，本发明涉及某些新的生物化学产生的化合物，即6-氨基己-2-烯酸，6-氨基己酸，以及由其制备的己内酰胺以及由这样的生物化学产生的化合物或己内酰胺制成的尼龙-6和其它衍生物 。