公开(公告)号：EP2305805B1

公开(公告)日：2015-11-11

申请号：EP09773487.5

申请日：2009-06-30

申请人： Daiichi Sankyo Company, Limited

发明人： KOIZUMI, Makoto ,  HIROTA, Yasuhide

IPC分类号： C12N15/113 ,  A61K31/713

CPC分类号： C12N15/111 ,  A61K31/713 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/343 ,  C12N2320/51 ,  C12N2310/3521

摘要： [Problem to be Solved] It is intended to provide a double-stranded polynucleotide that is resistant to RNase and has RNA interference effect, etc. [Solution] The present invention provides a double-stranded polynucleotide consisting of sense and antisense strands consisting of polynucleotides comprising a nucleotide unit of DNAs and 2'-O-methyl RNAs alternately combined.

公开(公告)号：EP2495323A1

公开(公告)日：2012-09-05

申请号：EP10826845.9

申请日：2010-10-29

申请人： Daiichi Sankyo Company, Limited

发明人： KOIZUMI, Makoto ,  HIROTA, Yasuhide

IPC分类号： C12N15/113 ,  A61K31/713 ,  A61K48/00 ,  A61P43/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1135 ,  A61K31/713 ,  C12N15/1136 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/344 ,  C12N2310/3521

摘要： The present invention provides a double-stranded polynucleotide having a sense strand polynucleotide consisting of a nucleotide sequence complementary to a target sequence in a target gene, and an antisense strand polynucleotide having a nucleotide sequence complementary to the sense strand polynucleotide, wherein an aryl or heteroaryl compound is bound to a phosphate group at the 5'-end of the antisense strand polynucleotide.

摘要翻译： 本发明提供了具有与目标基因中的靶序列互补的核苷酸序列组成的有义链多核苷酸的双链多核苷酸和具有与有义链多核苷酸互补的核苷酸序列的反义链多核苷酸，其中，芳基或杂芳基 化合物与反义链多核苷酸的5'末端的磷酸基结合。

公开(公告)号：EP2647713B1

公开(公告)日：2018-02-21

申请号：EP11845160.8

申请日：2011-12-01

申请人： Daiichi Sankyo Company, Limited

发明人： KOIZUMI, Makoto, ,  HIROTA, Yasuhide ,  NAKAYAMA, Makiko ,  IKEDA, Mika

IPC分类号： C12N15/11 ,  C12N15/113 ,  A61K31/7088 ,  A61K48/00 ,  C07C37/00

CPC分类号： C12N15/113 ,  A61K31/7088 ,  C07C235/08 ,  C07C235/34 ,  C07C235/48 ,  C07C235/56 ,  C12N15/111 ,  C12N2310/3183 ,  C12N2310/531 ,  C12N2320/51

摘要： It is intended to provide a polynucleotide that is resistant to RNase and has an RNA interference effect, etc. The present invention provides a single-stranded polynucleotide that is derived from a double-stranded polynucleotide comprising a sense strand polynucleotide corresponding to a target gene, and an antisense strand polynucleotide having a nucleotide sequence complementary to the sense strand polynucleotide, and has a structure in which the 5'-end of the antisense strand and the 3'-end of the sense strand are linked via a phenyl group-containing linker to form a phosphodiester structure at each of these ends.

公开(公告)号：EP2647713A1

公开(公告)日：2013-10-09

申请号：EP11845160.8

申请日：2011-12-01

申请人： Daiichi Sankyo Company, Limited

发明人： KOIZUMI, Makoto, ,  HIROTA, Yasuhide ,  NAKAYAMA, Makiko ,  IKEDA, Mika

IPC分类号： C12N15/113 ,  A61K31/7088 ,  A61K48/00 ,  C07C37/00

CPC分类号： C12N15/113 ,  A61K31/7088 ,  C07C235/08 ,  C07C235/34 ,  C07C235/48 ,  C07C235/56 ,  C12N15/111 ,  C12N2310/3183 ,  C12N2310/531 ,  C12N2320/51

摘要： It is intended to provide a polynucleotide that is resistant to RNase and has an RNA interference effect, etc. The present invention provides a single-stranded polynucleotide that is derived from a double-stranded polynucleotide comprising a sense strand polynucleotide corresponding to a target gene, and an antisense strand polynucleotide having a nucleotide sequence complementary to the sense strand polynucleotide, and has a structure in which the 5'-end of the antisense strand and the 3'-end of the sense strand are linked via a phenyl group-containing linker to form a phosphodiester structure at each of these ends.

摘要翻译： 旨在提供对RNA酶具有抗性并具有RNA干扰作用等的多核苷酸。本发明提供衍生自包含对应于靶基因的有义链多核苷酸的双链多核苷酸的单链多核苷酸， 和具有与有义链多核苷酸互补的核苷酸序列的反义链多核苷酸，并且具有其中反义链的5'末端和有义链的3'-末端通过含苯基的接头连接的结构 以在这些端部的每一个处形成磷酸二酯结构。

公开(公告)号：EP2305805A1

公开(公告)日：2011-04-06

申请号：EP09773487.5

申请日：2009-06-30

申请人： Daiichi Sankyo Company, Limited

发明人： KOIZUMI, Makoto ,  HIROTA, Yasuhide

IPC分类号： C12N15/09 ,  A61K31/713 ,  A61K48/00 ,  A61P43/00

CPC分类号： C12N15/111 ,  A61K31/713 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/343 ,  C12N2320/51 ,  C12N2310/3521

摘要： [Problem to be Solved]
It is intended to provide a double-stranded polynucleotide that is resistant to RNase and has RNA interference effect, etc.
[Solution]
The present invention provides a double-stranded polynucleotide consisting of sense and antisense strands consisting of polynucleotides comprising a nucleotide unit of DNAs and 2'-O-methyl RNAs alternately combined.

摘要翻译： [待解决的问题]旨在提供对RNA酶具有抗性并具有RNA干扰作用等的双链多核苷酸。[溶液]本发明提供了由多核苷酸组成的有义链和反义链组成的双链多核苷酸 包括交替组合的DNA和2'-O-甲基RNA的核苷酸单元。