公开(公告)号：EP3031801A1

公开(公告)日：2016-06-15

申请号：EP14834394.0

申请日：2014-03-27

申请人： Dalian Institute Of Chemical Physics Chinese Academy of Sciences

发明人： YANG, Ling ,  CUI, Jingnan ,  GE, Guangbo ,  LIU, Zhaoming ,  FENG, Lei

IPC分类号： C07D221/14 ,  C09K11/06 ,  G01N21/64

CPC分类号： C12Q1/46 ,  C07D221/14 ,  C09B57/08 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1029 ,  G01N33/582 ,  G01N2333/765

摘要： A pseudo-esterase activity -based fluorescent probe for specific detection of albumin and its applications. This probe is based on human serum albumin's pseudo-esterase activity, that is to say, under the condition of physiological pH value, the carboxylic ester bond of the probe (λex = 342 nm, λem = 416 nm) could be selectively cleaved by human serum albumin (HSA) to release the 4-hydroxy-1,8-naphthalimide fluorophore, while the dibenzoyl group covalently bound to the amino acid residues of HSA. The fluorescence emission spectrum of the product is significantly different from that of the substrate molecule (λex = 452 nm, λe m = 564 nm), and according to the fluorescence intensity of substrate and product we can detect the content of HSA in the system. This probe can be used for the determination of the absolute content of albumin in biological samples with the advantage of high accuracy, sensitivity, little influence from the environment and it is compatible with a variety of endogenous substances, exogenous drugs, and surfactant and so on.

摘要翻译： 用于特异性检测白蛋白的基于假酯酶活性的荧光探针及其应用。 该探针基于人血清白蛋白的假酯酶活性，也就是说，在生理pH值的条件下，探针的羧酸酯键（»ex = 342nm，»em = 416nm）可以选择性地裂解 通过人血清白蛋白（HSA）释放4-羟基-1,8-萘二甲酰亚胺荧光团，而二苯甲酰基共价结合到HSA的氨基酸残基。 产物的荧光发射光谱与底物分子（»ex = 452 nm，»em = 564 nm）的荧光发射光谱显着不同，根据底物和产物的荧光强度，我们可以检测系统中HSA的含量 。 该探针可用于测定生物样品中白蛋白的绝对含量，具有精度高，灵敏度高，环境影响小等优点，与各种内源性物质，外源药物和表面活性剂等相容 。