公开(公告)号：EP3166391A1

公开(公告)日：2017-05-17

申请号：EP15739713.4

申请日：2015-07-01

申请人： E. I. du Pont de Nemours and Company ,  Pioneer Hi-Bred International, Inc.

发明人： DJUKANOVIC, Vesna ,  JONES, Spencer Charles ,  LIU, Zhan-Bin ,  LASSNER, Michael ,  LYZNIK, Leszek, Aleksander

IPC分类号： A01H1/02 ,  C12N15/113 ,  C12N15/82

CPC分类号： C12N15/8275 ,  A01H1/02 ,  A01H4/008 ,  C12N15/8213 ,  C12N15/8241 ,  C12N2310/20 ,  C12Y301/00

摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.

摘要翻译： 本公开包括在phosphonomethylglycines家族的除草剂的生产突变植物抗性，例如 草甘膦。 提供了用于在细胞中编辑感兴趣的核苷酸序列采用导向的多核苷酸/ CAS核酸内切酶系统，worin中科院内切核酸酶通过导向的多核苷酸导入到识别和任选的特定靶位点到引入双海滩断组合物和方法 细胞的基因组中。 要被编辑的感兴趣的核苷酸序列可以位于内或在靶位点外也通过将CAS核酸内切酶识别。 更具体地，组合物和方法提供了用于在细胞烯醇丙酮莽草酸-3-磷酸合酶（EPSPS）的核苷酸序列的编辑。 所述方法和组合物使用的多核苷酸导/ CAS核酸内切酶在系统有效的系统，以提供用于编辑EPSPS细胞内的基因组中的核苷酸序列。 因此，提供了组合物和方法用于生产草甘膦耐性植物细胞，植物外植体，种子和谷物。