公开(公告)号：EP3611268A1

公开(公告)日：2020-02-19

申请号：EP19199945.7

申请日：2014-08-20

申请人： E. I. du Pont de Nemours and Company ,  Pioneer Hi-Bred International, Inc.

发明人： CIGAN, Andrew ,  FALCO, Saverio Carl ,  GAO, Huirong ,  LI, Zhongsen ,  LIU, Zhan-Bin ,  LYZNIK, L. Aleksander ,  SHI, Jinrui ,  SVITASHEV, Sergei ,  YOUNG, Joshua K.

IPC分类号： C12N15/82 ,  C12N15/113

摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

公开(公告)号：EP3036332B1

公开(公告)日：2020-06-24

申请号：EP14761477.0

申请日：2014-08-20

申请人： E. I. du Pont de Nemours and Company ,  Pioneer Hi-Bred International, Inc.

发明人： CIGAN, Andrew ,  FALCO, Saverio Carl ,  GAO, Huirong ,  LI, Zhongsen ,  LIU, Zhan-Bin ,  LYZNIK, L. Aleksander ,  SHI, Jinrui ,  SVITASHEV, Sergei ,  YOUNG, Joshua K.

IPC分类号： C12N15/82

公开(公告)号：EP3036332A1

公开(公告)日：2016-06-29

申请号：EP14761477.0

申请日：2014-08-20

申请人： E. I. du Pont de Nemours and Company ,  Pioneer Hi-Bred International, Inc.

发明人： CIGAN, Andrew ,  FALCO, Saverio Carl ,  GAO, Huirong ,  LI, Zhongsen ,  LIU, Zhan-Bin ,  LYZNIK, L. Aleksander ,  SHI, Jinrui ,  SVITASHEV, Sergei ,  YOUNG, Joshua K.

IPC分类号： C12N15/82

摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.

摘要翻译： 提供组合物和方法用于植物或植物细胞基因组中靶序列的基因组修饰。 方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。 还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于细胞或生物体基因组中的核苷酸序列进行基因组修饰，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法 的细胞或生物体。 一旦确定了基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。 还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。 还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。