公开(公告)号：EP3260465A1

公开(公告)日：2017-12-27

申请号：EP17167592.9

申请日：2006-06-06

申请人： ESBATech, an Alcon Biomedical Research Unit LLC

发明人： EWERT, Stefan ,  BARBERIS, Alcide ,  URECH, David M. ,  AUF DER MAUR, Adrian ,  LICHTLEN, Peter

IPC分类号： C07K16/24 ,  A61K39/395 ,  G01N33/53 ,  C12N15/13 ,  A61P37/00 ,  A61P29/00

CPC分类号： C07K16/241 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/522 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94

摘要： The present invention relates to particularly stable and soluble scFv antibodies and Fab fragments specific for TNFa, which comprise specific light chain and heavy chain sequences that are optimized for stability, solubility, in vitro and in vivo binding of TNFa, and low immunogenicity. Said antibodies are designed for the diagnosis and/or treatment of TNFa-related disorders. The nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, methods for isolating them and the use of said antibodies in medicine are also disclosed.

摘要翻译： 本发明涉及对TNFα特异的特别稳定和可溶的scFv抗体和Fab片段，其包含针对TNFα的稳定性，溶解性，体外和体内结合以及低免疫原性而优化的特定轻链和重链序列。 所述抗体被设计用于诊断和/或治疗TNFα相关病症。 还公开了用于表达本发明重组抗体的核酸，载体和宿主细胞，分离它们的方法以及所述抗体在药物中的用途。