公开(公告)号：EP2032690A2

公开(公告)日：2009-03-11

申请号：EP07725549.5

申请日：2007-05-25

申请人： Eberhard-Karls-Universität Tübingen

发明人： LEMBERT, Nicolas

IPC分类号： C12N5/06

CPC分类号： C12N5/0676 ,  C12N2509/00

摘要： The invention relates to a method for the in vitro preparation of living cells and/or cell accumulations from tissue and/or organs. According to said method, tissue and/or organic material containing the relevant cells is/are first prepared, the tissue is then treated to obtain at least partially fragmented and/or degraded tissue and/or organ material, said treatment being carried out in a container. In a subsequent step, a liquid medium is added to the tissue material/organ material from the previous step, to elute parts of the tissue and/or organ material from the container into a bag of the cell processor, the container and the bag being connected in such a way that they form a sealed system. Finally, the bag is centrifuged in the cell processor, the supernatant liquid is removed to obtain a concentrate containing the relevant cells and the concentrate is purified in the cell processor using a density gradient.