公开(公告)号：EP1382965B1

公开(公告)日：2005-11-30

申请号：EP02720570.7

申请日：2002-04-23

申请人： EIKEN KAGAKU KABUSHIKI KAISHA ,  Nagamune, Teruyuki

发明人： NAGAMUNE, Teruyuki ,  UEDA, Hiroshi ,  OHIRO, Yoshiyuki, c/o Eiken Kagaku Kabushiki K. ,  SHIBATA, Norio, c/o Eiken Kagaku Kabushiki Kaisha

IPC分类号： G01N33/542

CPC分类号： G01N33/54306 ,  G01N33/533 ,  G01N33/542 ,  G01N33/582

摘要： For highly accurate and sensitive measurement using an energy transfer phenomenon in a measurement system for measuring the concentration of a substance, a method for controlling the spatial arrangement of an energy donor and a receptor in a reaction composite, and a measurement system using the method. Two kinds of substances i.e., first and second substances each having an affinity to the substance to be measured are labeled with reporters a combination causing an energy transfer, Reagents which are labeled third and fourth substances having weak affinities to the respective labeled first and second substances, and a sample are mixed to form a reaction composite. Since the substances having the weakly affinities in the reaction composite are bonded because of the week affinities, the substances spatially approach each other. This state is stably held, so that a more efficient energy transfer occurs.

公开(公告)号：EP1382965A1

公开(公告)日：2004-01-21

申请号：EP02720570.7

申请日：2002-04-23

申请人： Eiken Kagaku Kabushiki Kaisha ,  Nagamune, Teruyuki

发明人： NAGAMUNE, Teruyuki ,  UEDA, Hiroshi ,  OHIRO, Yoshiyuki, c/o Eiken Kagaku Kabushiki K. ,  SHIBATA, Norio, c/o Eiken Kagaku Kabushiki Kaisha

IPC分类号： G01N33/542

CPC分类号： G01N33/54306 ,  G01N33/533 ,  G01N33/542 ,  G01N33/582

摘要： The present invention provides, in the use of the energy transfer phenomenon in a detection system for the measurement of the concentration of a material, a method for controlling the spatial arrangement of an energy donor and an energy acceptor in a reaction complex in order to carry out the measurement with good precision and high sensitivity, and a measurement system using the method.
Two types of material having affinity for the subject material to be measured are respectively labeled with a combination of reporters that give rise to energy transfer; those that have been thus obtained by labeling these materials are each further labeled with materials that have weak affinity for each other to give reagents, which are then mixed with a sample to give the reaction complex. Each of the materials is brought into spatial proximity by binding based on the affinity among the materials having weak affinity for each other in the reaction complex, and since that condition is stably maintained, a more efficient energy transfer occurs.

摘要翻译： 本发明提供了在用于测量材料浓度的检测系统中使用能量转移现象的方法，用于控制反应复合物中能量供体和能量受体的空间排列以便携带 高精度，高灵敏度的测量，以及使用该方法的测量系统。 对待测物体具有亲和性的两种材料分别用导致能量转移的报道分子的组合进行标记; 通过对这些材料进行标记而获得的那些每个都进一步用对彼此具有弱亲和力的材料进行标记以提供试剂，然后将这些试剂与样品混合以提供反应复合物。 基于在反应复合物中彼此具有弱亲和性的材料之间的亲和力，通过结合将每种材料置于空间上接近，并且由于稳定地保持该条件，发生更有效的能量转移。