公开(公告)号：EP1402050A2

公开(公告)日：2004-03-31

申请号：EP02740652.9

申请日：2002-05-27

申请人： F. HOFFMANN-LA ROCHE AG

发明人： HOESS, Eva ,  MEIER, Thomas ,  PESTLIN, Gabriele ,  POPP, Friedrich ,  REICHERT, Klaus ,  SCHMUCK, Rainer ,  SCHNEIDINGER, Bernd ,  SEIDEL, Christoph ,  TISCHER, Wilhelm

IPC分类号： C12P21/02 ,  C12N15/62 ,  C07K14/16 ,  C07K14/155

CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12P21/02

摘要： A process for the production of an antifusogenic peptide of a length of about 10 to 50 amino acids in a prokaryotic host cell, characterized in that, under such conditions that inclusion bodies of said non-fusion antifusogenic peptide or said fusion peptide are formed, a) in said host cell there is expressed a nucleic acid encoding said antifusogenic peptide as a non-fusion peptide or encoding a fusion peptide of a length of about 14 to 70 amino acids consisting of said antifusogenic peptide N-terminally linked to a further peptide of a length of about 4 to 30 amino acids; b) said host cell is cultivated; c) said inclusion bodies are recovered and solubilized; d) in the case of said fusion peptide said antifusogenic peptide is cleaved off from said further peptide; and e) said antifusogenic peptide is isolated.

公开(公告)号：EP1402050B1

公开(公告)日：2014-10-29

申请号：EP02740652.9

申请日：2002-05-27

申请人： F. Hoffmann-La Roche AG

发明人： HOESS, Eva ,  MEIER, Thomas ,  PESTLIN, Gabriele ,  POPP, Friedrich ,  REICHERT, Klaus ,  SCHMUCK, Rainer ,  SCHNEIDINGER, Bernd ,  SEIDEL, Christoph ,  TISCHER, Wilhelm

IPC分类号： C12P21/02 ,  C12N15/62 ,  C07K14/12 ,  C07K14/135 ,  C07K14/16

CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12P21/02