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1.发明公开PROCESS FOR THE MONO- AND BI-SIALYLATION OF GLYCOPROTEINS EMPLOYING N-TERMINALLY TRUNCATED BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE MUTANTS 审中-公开MONO的方法和糖蛋白WITH BISIALYLIERUNG N-末端CONDENSEDβ-半乳糖苷-ALPHA-2,6-唾液酸转移酶突变体
公开(公告)号：EP3017057A1
公开(公告)日：2016-05-11
申请号：EP14736379.0
申请日：2014-07-03
发明人： CZABANY, Tibor , ENGEL, Alfred , GREIF, Michael , JUNG, Christine , LULEY, Christiane , MALIK, Sebastian , MUELLER, Rainer , NIDETZKY, Bernd , RIBITSCH, Doris , SCHMOELZER, Katharina , SCHWAB, Helmut , SOBEK, Harald , SUPPMANN, Bernhard , THOMANN, Marco , ZITZENBACHER, Sabine
摘要： The present disclosure is directed to the use of certain glycosyltransferase variants having N-terminal truncation deletions. It was found that the combination of two different truncation variants of human β-galactoside-α-2,6-sialyltransferase I (hST6Gal-I) exhibited different specific sialyltransferase enzymatic activities. In one example, under conditions wherein the first variant Δ89 hST6Gal-I catalyzed formation of bi-sialylated target molecules the second variant Δ108 hST6Gal-I catalyzed formation of mono-sialylated target molecules. Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating in a quantitatively controlled manner terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.
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公开(公告)号：EP3017041A1
公开(公告)日：2016-05-11
申请号：EP14735569.7
申请日：2014-07-03
发明人： CZABANY, Tibor , ENGEL, Alfred , GREIF, Michael , JUNG, Christine , LULEY, Christiane , MALIK, Sebastian , MUELLER, Rainer , NIDETZKY, Bernd , RIBITSCH, Doris , SCHMOELZER, Katharina , SCHWAB, Helmut , SOBEK, Harald , SUPPMANN, Bernhard , THOMANN, Marco , ZITZENBACHER, Sabine
IPC分类号： C12N9/10
摘要： The present disclosure is directed to glycosyltransferase variants having N-terminal truncation deletions. Contrary to previous findings certain truncations comprising the conserved amino acid motif ("QVWxKDS") were found to be compatible with glycosyltransferase enzymatic activity, particularly in a human sialyltransferase (hST6Gal-I). Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.
摘要翻译： 本公开涉及具有N-末端截短缺失的糖基转移酶变体。 与以前的发现相反，发现包含保守氨基酸基序（“QVWxKDS”）的某些截短与糖基转移酶酶活性相容，特别是在人唾液酸转移酶（hST6Gal-1）中。 因此，所公开的是哺乳动物糖基转移酶的变体，编码它们的核酸，用于重组产生哺乳动物糖基转移酶变体的方法和手段及其用途，特别是作为糖蛋白的部分的聚糖末端受体基团（如免疫球蛋白）的唾液酸化。
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公开(公告)号：EP3017057B1
公开(公告)日：2019-10-02
申请号：EP14736379.0
申请日：2014-07-03
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公开(公告)号：EP3017041B1
公开(公告)日：2019-11-06
申请号：EP14735569.7
申请日：2014-07-03
发明人： CZABANY, Tibor , ENGEL, Alfred , GREIF, Michael , JUNG, Christine , LULEY, Christiane , MALIK, Sebastian , MUELLER, Rainer , NIDETZKY, Bernd , RIBITSCH, Doris , SCHMOELZER, Katharina , SCHWAB, Helmut , SOBEK, Harald , SUPPMANN, Bernhard , THOMANN, Marco , ZITZENBACHER, Sabine
IPC分类号： C12N9/10
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