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1.发明授权
公开(公告)号：EP0514509B1
公开(公告)日：2000-09-20
申请号：EP91920356.2
申请日：1991-11-28
发明人： CERQUEIRA DE ALMEIDA, Elza Carmen , KRIEGER, Marco Aurelio, Rua Desembargador Antonio , GOLDENBERG, Samuel
IPC分类号： G01N33/569
CPC分类号： G01N33/535 , G01N33/56905
摘要： This patent deals with the use of antigens conjugated to an enzymatic activity for the detection of immune-complexes against antigenic mixtures containing conjugated antigen, hence allowing the detection of antibodies even with low titre, through the development of a color reaction. The method is based on the specificity of antibodies and can be used for the diagnosis of any infectious disease with the condition of using the appropriate conjugated antigen. The diagnosis kits for Chagas' disease make use of the recombinant Trypanosoma cruzi antigens described in the patent application PCT/BR/91/00003, (Brazilian application number 9001479, of MAR, 30, 1990), and are indicated for the immunological diagnosis of Chagas' disease using either ELISA or the antigens immobilized to solid supports such as nitrocellulose, nylon, polyvinyl, polycarbonate and polyallomer.
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2.发明公开
公开(公告)号：EP0514509A1
公开(公告)日：1992-11-25
申请号：EP91920356.0
申请日：1991-11-28
发明人： CERQUEIRA DE ALMEIDA, Elza Carmen , KRIEGER, Marco Aurelio, Rua Desembargador Antonio , GOLDENBERG, Samuel
IPC分类号： G01N33
CPC分类号： G01N33/535 , G01N33/56905
摘要： On décrit l'utilisation d'antigènes conjugués à une activité enzymatique pour la détection de complexes immuns dressés contre les mélanges antigéniques qui renferment des antigènes conjugués, ce qui permet la détection même des anticorps à faible titre grâce à l'élaboration d'une réaction colorée. Le procédé est basé sur la spécificité des anticorps et peut servir au diagnostic de toutes les maladies infectieuses à condition que l'on se serve de l'antigène conjugué approprié. Les nécessaires destinés au diagnostic de la maladie de Chagas utilisent les antigènes recombinés de Trypanosoma cruzi décrits dans la demande de brevet PCT/BR 91/00003 (demande brésilienne numéro 9001479 du 30 mars 1990), et sont indiqués dans le diagnostic immunologique de la maladie de Chagas à l'aide soit de la technique ELISA soit des antigènes immobilisés sur des supports solides tels que la pyroxyline, le nylon, le polyvinyle, le polycarbonate et le polyallomère.
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3.发明公开
公开(公告)号：EP0486627A1
公开(公告)日：1992-05-27
申请号：EP91906678.0
申请日：1991-04-01
发明人： GOLDENBERG, Samuel , KRIEGER, Marco Aurelio, Rua Desembargador Antonio , CERQUEIRA DE ALMEIDA, Elza Carmen
CPC分类号： C07K14/44 , A61K38/00 , Y02A50/414
摘要： This patent deals with the use of Trypanosoma cruzi antigens obtained using recombinant DNA techniques, or synthetic peptides derived from these antigens, and their use in the immunological diagnosis of Chagas Disease using either ELISA, or western blot, or dot-blot, or hemagglutination, or agglutination, or monoclonal antibodies, or radioimmunoassay. The antigens or synthetic peptides are characterized by displaying the following aminoacid sequences: Ag1: valine (or alanine), alanine, glutamic acid, alanine (orthreonine), glutamic acid, lysine, glutamine, lysine (or arginine), alanine, alanine, glutamic acid, alanine (or serine), threonine (or methionine or alanine) and lysine. Ag2: methionine, glutamic acid, glutamine, glutamic acid arginine, arginine, glutamine, leucine, leucine, glutamic acid, lysine, aspartic acid, proline, arginine, arginine, asparagine, alanine, lysine, glutamic acid, isoleucine alanine, alanine, leucine, glutamic acid, glutamic acid, serine, methionine, asparagine, alanine, arginine, alanine, glutamine, glutamic acid, leucine, alanine, arginine, glutamic acid, lysine, lysine, leucine, arginine, aspartic acid, arginine, alanine, phenylalanine, leucine, aspartic acid, glutamine, lysine, proline, glutamic acid, arginine, valine, proline, leucine, alanine, aspartic acid, valine, proline, leucine, aspartic acid, aspartic acid, aspartic acid, serine, aspartic acid, phenylalanine, valine and alanine.
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