公开(公告)号：EP1692295A4

公开(公告)日：2008-10-01

申请号：EP04813931

申请日：2004-12-08

申请人： GENENCOR INT ,  DOW CORNING

发明人： BOND RISHA ,  JEWETT MICHAEL C ,  MCAULIFFE JOSEPH C ,  WARD II DONALD E

IPC分类号： C12N11/14 ,  C07K17/04 ,  C07K17/14 ,  C11D3/386 ,  C12N9/54 ,  C12N11/04 ,  C12N11/08 ,  C12P20060101 ,  G01N33/544 ,  G01N33/552

CPC分类号： C12N9/54 ,  C12N11/08 ,  C12N11/14

摘要： This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, d-hydroxylysine, and d-hydroxy-w-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.