PHENOL OXIDIZING ENZYME ENZYMES
    1.
    发明公开
    PHENOL OXIDIZING ENZYME ENZYMES 有权
    酚氧化酶及其用途

    公开(公告)号:EP1064359A2

    公开(公告)日:2001-01-03

    申请号:EP99912837.4

    申请日:1999-03-23

    CPC分类号: C11D3/38654 C12N9/0057

    摘要: Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.

    PHENOL OXIDIZING ENZYME ENZYMES
    2.
    发明授权
    PHENOL OXIDIZING ENZYME ENZYMES 有权
    酚氧化酶及其用途

    公开(公告)号:EP1064359B1

    公开(公告)日:2007-10-24

    申请号:EP99912837.4

    申请日:1999-03-23

    CPC分类号: C11D3/38654 C12N9/0057

    摘要: Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys chartarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.

    NOVEL ACID-STABLE AND THERMO-STABLE ENZYMES DERIVED FROM SULFOLOBUS SPECIES
    4.
    发明公开
    NOVEL ACID-STABLE AND THERMO-STABLE ENZYMES DERIVED FROM SULFOLOBUS SPECIES 失效
    从硫化类型新型酸性连续和热稳定的酶

    公开(公告)号:EP0871716A1

    公开(公告)日:1998-10-21

    申请号:EP95943520.0

    申请日:1995-07-07

    IPC分类号: C12N9 C12P19 C12R1

    CPC分类号: C12N9/2417 C12P19/14

    摘要: Novel acid-stable and thermo-stable enzymes having α-1,4 hydrolytic activity and a α-1,6 hydrolytic activity which are derived from strains of the genus Sulfolobus. These enzymes are capable of expressing high levels of α-1,4 hydrolytic activity, including the maximum α-1,4 hydrolytic activity thereof, at highly acidic pHs of between about 2.5 and about 4.5. These α-amylases are further capable of expressing high levels of α-1,4 hydrolytic activity, including the maximum α-1,4 hydrolytic activity thereof, at high temperatures of between about 90 °C and about 120 °C. Particularly disclosed herein are such enzymes which are derived from strains of the species S. acidocaldarius and, in particular, Sulfolobus acidocaldarius DSM 639. Modified starch degradation (liquefaction and saccharification) processes using these novel enzymes are also disclosed herein.