公开(公告)号：EP1309677B1

公开(公告)日：2008-07-09

申请号：EP01957519.0

申请日：2001-08-10

申请人： GENENCOR INTERNATIONAL, INC.

发明人： DIAZ-TORRES, Maria, R. ,  SCHELLENBERGER, Volker ,  SELIFONOVA, Olga, V. ,  MORRISON, Thomas, B. ,  LEE, Edwin, W.

IPC分类号： C12N15/00

CPC分类号： C12N15/902 ,  C12N15/102 ,  C12N15/90

摘要： The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.

公开(公告)号：EP1309677B2

公开(公告)日：2012-04-11

申请号：EP01957519.0

申请日：2001-08-10

申请人： GENENCOR INTERNATIONAL, INC.

发明人： DIAZ-TORRES, Maria, R. ,  SCHELLENBERGER, Volker ,  SELIFONOVA, Olga, V. ,  MORRISON, Thomas, B. ,  LEE, Edwin, W.

IPC分类号： C12N15/00

CPC分类号： C12N15/902 ,  C12N15/102 ,  C12N15/90

摘要： The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.

公开(公告)号：EP1309677A2

公开(公告)日：2003-05-14

申请号：EP01957519.0

申请日：2001-08-10

申请人： GENENCOR INTERNATIONAL, INC.

发明人： DIAZ-TORRES, Maria, R. ,  SCHELLENBERGER, Volker ,  SELIFONOVA, Olga, V. ,  MORRISON, Thomas, B. ,  LEE, Edwin, W.

IPC分类号： C12N15/00

CPC分类号： C12N15/902 ,  C12N15/102 ,  C12N15/90

摘要： The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.