公开(公告)号：EP0755442B1

公开(公告)日：2002-10-09

申请号：EP95919388.9

申请日：1995-04-28

申请人： GENENCOR INTERNATIONAL, INC.

发明人： AEHLE, Wolfgang ,  GERRITSE, Gijsbert ,  LENTING, Hermanus, Bernardus, Maria

IPC分类号： C12N9/20 ,  C11D3/386

CPC分类号： C12N9/20 ,  C11D3/38627 ,  Y10S435/874

摘要： The present invention provides mutant lipases which retain lipase activity, but have improved surfactant resistance and are thus highly advantageous for use in detergent compositions. Preferred lipases of this type include modified functional forms of a lipase of Pseudomonas alcaligenes having one or more amino acid substituents at or near the surface which effect interaction of the hydrophobic portions of surfactant molecules with initiation sites on the enzyme for surfactant denaturation.

公开(公告)号：EP1769069B1

公开(公告)日：2017-11-15

申请号：EP05758989.7

申请日：2005-07-07

申请人： DuPont Nutrition Biosciences ApS ,  Genencor International, Inc.

发明人： BERG, Casper, Tune ,  DERKX, Patrick, M., F. ,  FIORESI, Carol ,  GERRITSE, Gijsbert ,  KELLET-SMITH, Anja, Hemmingen ,  KRAGH, Karsten, Matthias ,  LIU, Wei ,  SHAW, Andrew ,  SØRENSEN, Bo, Spange ,  THOUDAHL, Charlotte, Refdahl

IPC分类号： C12N9/28 ,  C12P19/14 ,  A21D8/04

CPC分类号： C12Y302/0106 ,  A21D8/042 ,  A23K20/189 ,  A23L7/107 ,  A23L29/35 ,  A23V2002/00 ,  C12N9/2417 ,  A23V2300/21

公开(公告)号：EP0755442A1

公开(公告)日：1997-01-29

申请号：EP95919388.0

申请日：1995-04-28

申请人： GENENCOR INTERNATIONAL INC.

发明人： AEHLE, Wolfgang ,  GERRITSE, Gijsbert ,  LENTING, Hermanus, Bernardus, Maria

IPC分类号： C12N15 ,  C11D3 ,  C12N9

CPC分类号： C12N9/20 ,  C11D3/38627 ,  Y10S435/874

摘要： The present invention provides mutant lipases which retain lipase activity, but have improved surfactant resistance and are thus highly advantageous for use in detergent compositions. Preferred lipases of this type include modified functional forms of a lipase of Pseudomonas alcaligenes having one or more amino acid substituents at or near the surface which effect interaction of the hydrophobic portions of surfactant molecules with initiation sites on the enzyme for surfactant denaturation.

公开(公告)号：EP1907538A1

公开(公告)日：2008-04-09

申请号：EP06755730.6

申请日：2006-07-07

申请人： Danisco A/S, Danisco Intellectual Capital ,  GENENCOR INTERNATIONAL, INC.

发明人： BERG, Casper, Tune ,  DERKX, Patrick, Maria, Franciscus ,  FIORESI, Carol ,  GERRITSE, Gijsbert ,  KELLET-SMITH, Anja, Hemmingen ,  KRAGH, Karsten, Matthias ,  LIU,Wei ,  SHAW, Andrew ,  SØRENSEN, Bo, Spange ,  THOUDAHL, Charlotte, Refdahl

IPC分类号： C12N9/28

CPC分类号： A21D8/042 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1 , uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

公开(公告)号：EP1649006A2

公开(公告)日：2006-04-26

申请号：EP04786045.7

申请日：2004-07-07

申请人： GENENCOR INTERNATIONAL, INC. ,  DANISCO A/S

发明人： BERG, Casper, Tune ,  BOJSEN, Kirsten ,  DERKX, Patrick, M.,.F. ,  FIORESI, Carol ,  KRAGH, Karsten, M. ,  LIU, Wei ,  SHAW, Andrew ,  THOUDAHL, Charlotte, R. ,  GERRITSE, Gijsbert

IPC分类号： C12N1/00

CPC分类号： C12N9/2417 ,  A21D8/042 ,  A23L29/06 ,  A23L29/35 ,  A23L33/17 ,  A23V2002/00 ,  A23V2300/21

摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.

公开(公告)号：EP1769069A2

公开(公告)日：2007-04-04

申请号：EP05758989.7

申请日：2005-07-07

申请人： DANISCO A/S ,  Genencor International, Inc.

发明人： BERG, Casper, Tune ,  DERKX, Patrick, M., F. ,  FIORESI, Carol ,  GERRITSE, Gijsbert ,  KELLET-SMITH, Anja, Hemmingen ,  KRAGH, Karsten, Matthias ,  LIU, Wei ,  SHAW, Andrew ,  SØRENSEN, Bo, Spange ,  THOUDAHL, Charlotte, Refdahl

IPC分类号： C12N9/28

CPC分类号： C12Y302/0106 ,  A21D8/042 ,  A23K20/189 ,  A23L7/107 ,  A23L29/35 ,  A23V2002/00 ,  C12N9/2417 ,  A23V2300/21

摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.

摘要翻译： 我们描述了可从具有非麦芽糖苷外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽包含一个或多个选自以下的位置的氨基酸突变：121,161,223,146,157 ，158,198,229,303,306,309,316,353,26,70,105,188,272,339，参考SEQ ID NO：1所示的假单胞菌属的嗜酸性粒细胞外淀粉酶序列的位置编号。

公开(公告)号：EP0918851B1

公开(公告)日：2004-10-13

申请号：EP97938360.1

申请日：1997-08-15

申请人： GENENCOR INTERNATIONAL, INC.

发明人： GERRITSE, Gijsbert ,  QUAX, Wilhelmus, J.

IPC分类号： C12N15/00

CPC分类号： C07K14/21 ,  C12N9/20 ,  C12N15/78

摘要： A new expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

公开(公告)号：EP0918851A2

公开(公告)日：1999-06-02

申请号：EP97938360.0

申请日：1997-08-15

申请人： GENENCOR INTERNATIONAL INC.

发明人： GERRITSE, Gijsbert ,  QUAX, Wilhelmus, J.

IPC分类号： C07K14 ,  C12N9 ,  C12N15

CPC分类号： C07K14/21 ,  C12N9/20 ,  C12N15/78

摘要： An expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.