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1.发明公开Method for enhancing somatic hypermutation, gene conversion and class switch recombination 审中-公开Verfahren zurVerstärkungsomatischer Hypermutationen，Genkonversion und“Class-Switch”Rekombination
公开(公告)号：EP1780272A1
公开(公告)日：2007-05-02
申请号：EP05023503.5
申请日：2005-10-27
IPC分类号： C12N15/00
CPC分类号： C07K14/4702 , C07K16/00 , C12N15/1024
摘要： The invention relates to a method for enhancing somatic hypermutation, gene conversion and/or immunoglobulin class switch recombination in a cell by increasing the level of an E2A encoded protein in the cell. The invention also relates to cells and cell lines with an increased level of said activities.
摘要翻译： 本发明涉及通过增加细胞中E2A编码的蛋白质的水平来增强细胞体细胞超突变，基因转化和/或免疫球蛋白类转换重组的方法。 本发明还涉及具有增加的所述活性水平的细胞和细胞系。
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2.发明公开Method for genetic diversification in gene conversion active cells 审中-公开Methode zur genetischen Diversifikation in Zellen，in denen Genkonversion auftritt
公开(公告)号：EP1568765A1
公开(公告)日：2005-08-31
申请号：EP04004062.8
申请日：2004-02-23
IPC分类号： C12N5/06 , C12N15/01 , A01K67/027
CPC分类号： C07K16/00 , C12N15/1024
摘要： The invention relates to a modified lymphoid cell having gene conversion fully or partially replaced by hypermutation, wherein said cell has no deleterious mutations in genes encoding paralogues and analogues of the RAD51 protein, and wherein said cell is capable of directed and selective genetic diversification of a target nucleic acid by hypermutation or a combination of hypermutation and gene conversion. The invention also relates to a method for diversifying any transgenic target gene in said cell. Preferably, the target gene is integrated into the immunoglobulin light or heavy chain locus by targeted integration.
摘要翻译： 本发明涉及具有完全或部分被超突变替代的基因转化的修饰淋巴样细胞，其中所述细胞在编码RAD51蛋白的旁系同源物和类似物的基因中没有有害突变，并且其中所述细胞能够进行定向和选择性的遗传多样化 靶核酸通过超突变或超突变和基因转化的组合。 本发明还涉及用于使所述细胞中任何转基因靶基因多样化的方法。 优选地，靶基因通过靶向整合整合到免疫球蛋白轻链或重链基因座中。
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