公开(公告)号：EP3067416A4

公开(公告)日：2017-10-04

申请号：EP14860811

申请日：2014-08-25

申请人： GUANGZHOU INST BIOMED & HEALTH

发明人： LIU XINGGUO ,  PEI DUANQING ,  LIU JINGLEI ,  LIU XUEBIN ,  YAO DEYANG

IPC分类号： C12N5/10 ,  C12N5/071 ,  C12N5/0786 ,  C12N15/85 ,  C12N15/87

CPC分类号： C12N5/0645 ,  C12N15/87 ,  C12N2510/00

摘要： The present disclosure provides a method for producing a cell with exogenous mitochondria by obtaining synthetic mitochondria via introduction of exogenous mitochondrial DNA into mitochondria or empty mitochondrial shells, and incorporating the same into mammalian cells via endocytosis. As such, effective functionality of exogenous mitochondria in cells is realized. The synthetic mitochondrial DNA genes introduced according to the present disclosure can be stably expressed and effectively passaged. The method for introducing exogenous mitochondrial DNA into mammalian cells as disclosed herein may be used as a whole new mitochondrial molecular cloning means to perform site-directed mutagenesis, gene insertion, gene knockout, gene rearrangement, and the like in mitochondria. Therefore, any molecular cloning modification can be performed on a mammalian mitochondrial DNA, which is of great importance to therapeutic schemes of diseases derived from mitochondrial DNA mutations.

摘要翻译： 本公开提供了一种通过将外源线粒体DNA导入线粒体或空线粒体壳获得合成线粒体并通过胞吞作用将其掺入哺乳动物细胞中来产生具有外源线粒体的细胞的方法。 如此，实现了细胞中外源线粒体的有效功能。 根据本公开引入的合成线粒体DNA基因可以稳定表达并有效传代。 如本文所公开的用于将外源线粒体DNA导入哺乳动物细胞的方法可以用作整个新的线粒体分子克隆方法以在线粒体中进行定点诱变，基因插入，基因敲除，基因重排等。 因此，可以对哺乳动物线粒体DNA进行任何分子克隆修饰，这对线粒体DNA突变导致的疾病的治疗方案非常重要。