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    A CELL SURFACE TAG EXCHANGE (CSTE) SYSTEM FOR TRACING AND MANIPULATION OF CELLS DURING RECOMBINASE MEDIATED CASSETTE EXCHANGE INTEGRATION OF NUCLEIC ACID SEQUENCES TO ENGINEERED RECEIVER CELLS
    5.
    发明公开
    A CELL SURFACE TAG EXCHANGE (CSTE) SYSTEM FOR TRACING AND MANIPULATION OF CELLS DURING RECOMBINASE MEDIATED CASSETTE EXCHANGE INTEGRATION OF NUCLEIC ACID SEQUENCES TO ENGINEERED RECEIVER CELLS 审中-公开

    公开(公告)号：EP4296363A2

    公开(公告)日：2023-12-27

    申请号：EP23205633.3

    申请日：2019-07-09

    申请人： Genovie AB

    发明人： JARVIS, Reagan ,  PASE, Luke ,  HILL, Ryan

    IPC分类号： C12N15/90

    摘要： A combined system comprising two separate components, wherein the first component is a tag-exchange donor vector (TEDV) encoding a first cell surface tag (CST) exon flanked by a 3' intron fragment, and a gene of interest (GOI) in the antisense orientation, and the second component is an engineered cell containing within its genome a tag-exchange receiver site (TERS), encoding a second CST exon adjoined by a full intron sequence to an exon encoding a transmembrane domain, and also encoding a reporter gene in the antisense orientation, wherein paired recombinase mediated cassette exchange (RMCE) elements are included in the TEDV and TERS such that execution of RMCE between the TEDV and TERS results in exchange of the reporter element for the GOI encoded by the TEDV, and exchange of the first CST exon for the second CST exon, such that the derivative engineered cell now expresses the first CST and GOI, in place of the second CST and the reporter gene.

    A CELL SURFACE TAG EXCHANGE (CSTE) SYSTEM FOR TRACING AND MANIPULATION OF CELLS DURING RECOMBINASE MEDIATED CASSETTE EXCHANGE INTEGRATION OF NUCLEIC ACID SEQUENCES TO ENGINEERED RECEIVER CELLS
    7.
    发明公开
    A CELL SURFACE TAG EXCHANGE (CSTE) SYSTEM FOR TRACING AND MANIPULATION OF CELLS DURING RECOMBINASE MEDIATED CASSETTE EXCHANGE INTEGRATION OF NUCLEIC ACID SEQUENCES TO ENGINEERED RECEIVER CELLS 审中-实审

    公开(公告)号：EP4296363A3

    公开(公告)日：2024-03-20

    申请号：EP23205633.3

    申请日：2019-07-09

    申请人： Genovie AB

    发明人： JARVIS, Reagan ,  PASE, Luke ,  HILL, Ryan

    IPC分类号： C12N15/79 ,  C12N15/85

    摘要： A combined system comprising two separate components, wherein the first component is a tag-exchange donor vector (TEDV) encoding a first cell surface tag (CST) exon flanked by a 3' intron fragment, and a gene of interest (GOI) in the antisense orientation, and the second component is an engineered cell containing within its genome a tag-exchange receiver site (TERS), encoding a second CST exon adjoined by a full intron sequence to an exon encoding a transmembrane domain, and also encoding a reporter gene in the antisense orientation, wherein paired recombinase mediated cassette exchange (RMCE) elements are included in the TEDV and TERS such that execution of RMCE between the TEDV and TERS results in exchange of the reporter element for the GOI encoded by the TEDV, and exchange of the first CST exon for the second CST exon, such that the derivative engineered cell now expresses the first CST and GOI, in place of the second CST and the reporter gene.

    AN ENGINEERED MULTI-COMPONENT SYSTEM FOR IDENTIFICATION AND CHARACTERISATION OF T-CELL RECEPTORS AND T-CELL ANTIGENS
    8.
    发明公开
    AN ENGINEERED MULTI-COMPONENT SYSTEM FOR IDENTIFICATION AND CHARACTERISATION OF T-CELL RECEPTORS AND T-CELL ANTIGENS 审中-公开

    公开(公告)号：EP4332232A2

    公开(公告)日：2024-03-06

    申请号：EP23205652.3

    申请日：2017-11-07

    申请人： Genovie AB

    发明人： JARVIS, Reagan ,  HILL, Ryan ,  PASE, Luke

    IPC分类号： C12N15/85

    摘要： The present invention relates to a multicomponent system wherein a first component is an engineered antigen-presenting cell (eAPC) designated component A and a second component is a genetic donor vector, designated component C, for delivery of one or more ORFs encoding an analyte antigen-presenting complex (aAPX) and/or an analyte antigenic molecule (aAM), wherein component A
    a. Lacks endogenous surface expression of at least one family of aAPX and/or aAM and
    b. Contains at least one genomic receiver sites, designated component B for integration of at least one ORF encoding at least one aAPX and/or aAM, wherein the genomic receiver site component B is a synthetic construct designed for site directed homologous recombination.