利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.013 秒)

    EXPRESSION CONSTRUCTS AND METHODS FOR SELECTING HOST CELLS EXPRESSING POLYPEPTIDES
    3.
    发明公开
    EXPRESSION CONSTRUCTS AND METHODS FOR SELECTING HOST CELLS EXPRESSING POLYPEPTIDES 审中-公开
    VERFAHREN ZUR AUSWAHL VON POLYPEPTIEXEXPRIMIERENDEN WIRTSZELLEN表演表演

    公开(公告)号：EP3110950A1

    公开(公告)日：2017-01-04

    申请号：EP15707625.8

    申请日：2015-03-02

    申请人： Glenmark Pharmaceuticals S.A.

    发明人： AEBISCHER-GUMY, Christel ,  MORETTI, Pierre ,  BERTSCHINGER, Martin

    IPC分类号： C12N15/09

    CPC分类号： G01N33/56966 ,  C07K14/705 ,  C07K16/00 ,  C07K2317/31 ,  C07K2317/52 ,  C07K2317/622 ,  C12N15/1037 ,  C12N15/85 ,  C12N2830/42 ,  C12N2830/50 ,  C12P21/00 ,  G01N33/6854

    摘要： The invention relates to an expression construct for the soluble expression of polypeptides in host cells and for the expression of polypeptides on the surface of host cells, using alternative splicing. The amount of cell membrane expression of polypeptides such as antibodies, antibody fragments and bispecific antibodies can be correlated directly to the amount of soluble polypeptide expressed. In the case of bispecific antibodies, cell membrane expression of heterodimer and homodimer products can be correlated directly to the soluble expression of these products, thereby aiding selection of a desired producer clone.

    摘要翻译： 本发明涉及使用选择性剪接在宿主细胞中可溶性表达多肽并在宿主细胞表面上表达多肽的表达构建体。 多肽如抗体，抗体片段和双特异性抗体的细胞膜表达量可直接与表达的可溶性多肽的量相关。 在双特异性抗体的情况下，异二聚体和同二聚体产物的细胞膜表达可以直接与这些产物的可溶性表达相关，从而有助于选择所需的生产者克隆。