公开(公告)号：EP3875590A1

公开(公告)日：2021-09-08

申请号：EP18938531.3

申请日：2018-10-30

申请人： Green Earth Institute Co., Ltd.

发明人： YAMAMOTO, Keisuke ,  TSUCHISAKA, Atsunari ,  NAKANE, Shuhei ,  NAKAYASHIKI, Toru

IPC分类号： C12N15/60 ,  C12N9/88 ,  C12P7/18 ,  C12P7/42 ,  C12P7/50 ,  C12P11/00 ,  C12P13/00

摘要： A method for producing an α-keto acid includes a step of culturing a microorganism containing a gene encoding an enzyme that catalyzes an aldol reaction between pyruvic acid and a carbonyl compound in the presence of a pyruvic acid-supplying compound selected from pyruvic acid and saccharides, and a carbonyl compound selected from an aldehyde and a ketone to produce an α-keto acid.

公开(公告)号：EP4365219A1

公开(公告)日：2024-05-08

申请号：EP22832557.7

申请日：2022-03-31

申请人： DIC Corporation ,  Green Earth Institute Co., Ltd.

发明人： KONDO, Akihiro ,  OKA, Yasutaka ,  SASAMOTO, Akane ,  ARAI, Tatsuhiko ,  NAKAYASHIKI, Toru ,  MIURA, Satomi

IPC分类号： C08G73/10 ,  C08J3/12 ,  C08J3/24

CPC分类号： C08J3/12 ,  C08J3/24 ,  C08G73/10

摘要： Provided is an aspartic acid composition containing aspartic acid and an impurity, the impurity containing at least one selected from the group consisting of an amino acid other than aspartic acid, an organic acid, and salts of the amino acid other than aspartic acid and the organic acid, a content of the impurity being 0.02% by mass or more and 1.1% by mass or less. Also provided is a method for producing polysuccinimide, the method including performing a polymerization reaction of the aspartic acid using the aspartic acid composition. Also provided is a polysuccinimide composition produced by the method for producing polysuccinimide.

公开(公告)号：EP3954756A1

公开(公告)日：2022-02-16

申请号：EP19924352.8

申请日：2019-09-06

申请人： Green Earth Institute Co., Ltd.

发明人： NAKAYASHIKI, Toru

IPC分类号： C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N9/88 ,  C12N15/60 ,  C12P7/42 ,  C12P7/46 ,  C12P13/06 ,  C12P13/20

摘要： The present disclosure pertains to a genetically modified microorganism that satisfies some of specific requirements. The specific requirements include: (I) compared to a wild type microorganism, having reduced or inactivated succinate dehydrogenase activity or fumarate reductase activity; (II) compared to a wild type microorganism, having reduced or inactivated lactate dehydrogenase activity; (III) having a modified phosphoenolpyruvate carboxylase activity and thus showing resistance against the feedback inhibition by aspartic acid in wild type phosphoenolpyruvate carboxylase activity, or having an exogenous phosphoenolpyruvate carboxylase activity and thus showing higher resistance against the feedback inhibition by aspartic acid than wild type phosphoenolpyruvate carboxylase activity shown by a wild type microorganism; and (IV) compared to a wild type microorganism, having reduced or inactivated pyruvate : quinone oxidoreductase.