公开(公告)号：EP2778222B1

公开(公告)日：2017-03-22

申请号：EP13167122.4

申请日：2012-03-31

申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences

发明人： Pei, Duanqing ,  Wang, Tao

IPC分类号： C12N5/071 ,  C12N5/10

CPC分类号： C12N5/0662 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2501/602 ,  C12N2501/72 ,  C12N2506/13 ,  C12N2510/00

公开(公告)号：EP2778222A1

公开(公告)日：2014-09-17

申请号：EP13167122.4

申请日：2012-03-31

申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences

发明人： Pei, Duanqing ,  Wang, Tao

IPC分类号： C12N5/071 ,  C12N5/10

CPC分类号： C12N5/0662 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2501/602 ,  C12N2501/72 ,  C12N2506/13 ,  C12N2510/00

摘要： The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells, and more particularly, to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1a that modify histone. By utilizing Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method of the present invention further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.

摘要翻译： 本发明涉及提高多能干细胞诱导效率的方法，更具体地说，涉及通过利用修饰组蛋白的基因Jhdm1a来提高诱导多能干细胞的效率的方法。 通过利用Jhdm1a和干细胞诱导因子，本发明增加诱导多能干细胞的效率并增加诱导多能干细胞的质量。 干细胞诱导因子是Oct4和Klf4的组合，或Sox2，Oct4和Klf4的组合，或Oct4和Sox2以及Oct4的组合。 与不使用维生素C的情况相比，本发明的方法还包括将细胞暴露于维生素C，这进一步提高了诱导多能干细胞的效率。 通过使用较少的干细胞减少因子，本发明的方法降低潜在致癌性，获得高诱导效率，并提供能够进行种系传播的高质量诱导多能干细胞。