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公开(公告)号：EP1425384B1
公开(公告)日：2006-12-20
申请号：EP02776674.0
申请日：2002-09-13
申请人： HANS-KNÖLL-INSTITUT FÜR NATURSTOFF-FORSCHUNG E.V. , INSTITUT FÜR BIOPROZESS- UND ANALYSENMESSTECHNIK E.V. , INSTITUT FÜR PHYSIKALISCHE HOCHTECHNOLOGIE E.V.
发明人： MÜLLER, Peter-Jürgen , ROTH, Martin , GROTH, Ingrid , KUMMER, Christel , MARTIN, Karin , SCHROECKH, Volker , METZE, Josef , KÖHLER, Michael , HENKEL, Thomas , GASTROCK, Gunter , LEMKE, Karen , HILLIGER, Matthias
摘要： The invention relates to methods for individually separating microorganisms from a suspension or culture, parallel cultivation of individual cells and analyzing the metabolic performances thereof. The invention enables basic microbial operations such as media optimization, screening according to images of novel natural substances and special metabolic performances, qualitative and quantitative detection of the effects of nutrient substrates, effectors and active substances including the media optimization and selection of microorganism clones with specific properties from large populations according to mutagenesis, transformation, transfection and genetic processing in addition to the detection of microbial contaminations to be carried out. One advantage of the invention is that it can be applied when microorganisms having outstanding properties can be respectively obtained as individual cells or individual organisms from a large population and can be characterized as pure cultures or when the effect of influencing variables can be examined in many fully comparable cultures.
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公开(公告)号：EP1425384A2
公开(公告)日：2004-06-09
申请号：EP02776674.0
申请日：2002-09-13
申请人： HANS-KNÖLL-INSTITUT FÜR NATURSTOFF-FORSCHUNG e.V. , INSTITUT FÜR BIOPROZESS- UND ANALYSENMESSTECHNIK e.V. , INSTITUT FÜR PHYSIKALISCHE HOCHTECHNOLOGIE E.V.
发明人： MÜLLER, Peter-Jürgen , ROTH, Martin , GROTH, Ingrid , KUMMER, Christel , MARTIN, Karin , SCHROECKH, Volker , METZE, Josef , KÖHLER, Michael , HENKEL, Thomas , GASTROCK, Gunter , LEMKE, Karen , HILLIGER, Matthias
摘要： The invention relates to methods for individually separating microorganisms from a suspension or culture, parallel cultivation of individual cells and analyzing the metabolic performances thereof. The invention enables basic microbial operations such as media optimization, screening according to images of novel natural substances and special metabolic performances, qualitative and quantitative detection of the effects of nutrient substrates, effectors and active substances including the media optimization and selection of microorganism clones with specific properties from large populations according to mutagenesis, transformation, transfection and genetic processing in addition to the detection of microbial contaminations to be carried out. One advantage of the invention is that it can be applied when microorganisms having outstanding properties can be respectively obtained as individual cells or individual organisms from a large population and can be characterized as pure cultures or when the effect of influencing variables can be examined in many fully comparable cultures.
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公开(公告)号：EP0706646B1
公开(公告)日：1998-03-25
申请号：EP94922254.1
申请日：1994-07-02
申请人： INSTITUT FÜR MOLEKULARE BIOLOGIE E.V. , INSTITUT FÜR PHYSIKALISCHE HOCHTECHNOLOGIE E.V. , Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Berlin
发明人： EIGEN, Manfred , HENCO, Karsten , SCHOBER, Andreas , SCHWIENHORST, Andreas , KÖHLER, Michael , THÜRK, Marcel , GÜNTHER, Rolf , DÖRING, Michael
CPC分类号： B01L3/50255 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00319 , B01J2219/00378 , B01J2219/0043 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00659 , B01J2219/00689 , B01J2219/00704 , B01J2219/00722 , B01J2219/00725 , B01L3/5085 , C40B40/06 , C40B40/10 , G01N1/28 , G01N30/24 , G01N30/466 , G01N30/6095 , G01N30/74 , G01N2030/8813 , G01N2030/8827 , G01N2030/8831 , G01N2035/00237
摘要： Described is a device designed to hold a multiplicity of chemical and/or biological samples. The device has at least one mechanical micro-sample holder (50) with a lower surface (60) and, remote from it, an upper surface (58), the holder being divided into a multiplicity of recesses (52) with floors, the recesses (52) being produced using non-cutting techniques, in particular by etching. In order to produce apertures extending between the upper and lower surfaces of the sample holder, at least part of the floor (54) of each recess (52) is porous.
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