公开(公告)号：EP3145538A4

公开(公告)日：2017-12-06

申请号：EP15796798

申请日：2015-05-20

申请人： HRYZ BIOTECH CO ,  SYZ CELL THERAPY CO

发明人： MA YIFAN ,  ZHOU XIANGJUN ,  CHEN SHANG ,  CAI LINTAO ,  WANG CE ,  LIU PENG

IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61K47/26 ,  A61K47/34 ,  A61P35/00 ,  C12N5/0784 ,  C12N7/00

CPC分类号： A61K39/0011 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/5154 ,  A61K2039/55555 ,  C12N5/0639 ,  C12N7/00 ,  C12N2500/90 ,  C12N2501/05 ,  C12N2501/22 ,  C12N2501/2304 ,  C12N2730/10134

摘要： The present invention provides a method for preparing dendritic cell loaded with antigen, the method comprising the steps of adding serum-free cell culture medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and inter-leukin (IL)-4 into mononuclear cells, culturing in an incubator at 37° C. under 5% carbon dioxide for 5 days, adding target antigen wrapped cationic liposome and culturing for 8-24 hours to obtain target antigen loaded dendritic cell.