公开(公告)号：EP2561358A2

公开(公告)日：2013-02-27

申请号：EP11724450.9

申请日：2011-04-18

申请人： Hellenic Pasteur Institute ,  Tzartos, Socrates ,  Tzartos, John ,  Tzartou, Elissavet

发明人： TZARTOS, Socrates ,  TZARTOS, John ,  TZARTOU, Elissavet ,  TRAKAS, Nikolaos ,  STERGIOU, Christos ,  ZISIMOPOULOU, Paraskevi

IPC分类号： G01N33/53 ,  G01N33/68

CPC分类号： G01N33/564 ,  G01N2800/285

摘要： A method of detecting the presence of a target molecule in a biological sample from a subject, wherein the target molecule is capable of binding to a binding molecule, wherein the method comprises the steps of: a) subjecting the biological sample to a purification step to provide an enriched target molecule sample, wherein the purification step comprises: i) contacting binding molecules with the biological sample to enable binding of the target molecules to the binding molecules, and ii) removing target molecules from the binding molecules to provide the enriched target molecule sample; and b) subjecting the enriched target molecule sample to an assay to detect the presence of the target molecule.