公开(公告)号：EP1582581A2

公开(公告)日：2005-10-05

申请号：EP05003191.3

申请日：2005-02-15

申请人： Hitachi High-Technologies Corporation

发明人： Takaki, Masanori ,  Yamada, Kuriko ,  Deguch, Kisaburo ,  Nakagawa, Hiroaki ,  Nishimura, Shinichiro

IPC分类号： C12M1/40

CPC分类号： C12P19/18

摘要： The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.

摘要翻译： 本发明提供了当多个糖链连续反应时能够连续反应糖链的糖链合成器。 本发明的糖链合成装置包括含有各糖苷溶液的多个容器，含有各糖基转移酶的多个容器，以及含有水溶性聚合物的底物的反应器，上述糖核苷酸溶液和 糖基转移酶。 在本发明中，通过超滤塔将在反应器中得到的反应溶液中的成分分离，然后将反应产物返回到上述反应器中，以连续合成糖链。 尽管糖链是复杂的合成，但可以连续自动地进行这种合成。

公开(公告)号：EP1582581B1

公开(公告)日：2013-07-03

申请号：EP05003191.3

申请日：2005-02-15

申请人： Hitachi High-Technologies Corporation

发明人： Takaki, Masanori ,  Yamada, Kuriko ,  Deguchi, Kisaburo ,  Nakagawa, Hiroaki ,  Nishimura, Shinichiro

IPC分类号： C12M1/40

CPC分类号： C12P19/18

公开(公告)号：EP1582581A3

公开(公告)日：2007-03-07

申请号：EP05003191.3

申请日：2005-02-15

申请人： Hitachi High-Technologies Corporation

发明人： Takaki, Masanori ,  Yamada, Kuriko ,  Deguch, Kisaburo ,  Nakagawa, Hiroaki ,  Nishimura, Shinichiro

IPC分类号： C12M1/40

CPC分类号： C12P19/18

摘要： The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.