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    NUCLEIC ACID AMPLIFICATION METHOD
    2.
    发明授权
    NUCLEIC ACID AMPLIFICATION METHOD 有权

    公开(公告)号：EP2792743B1

    公开(公告)日：2018-08-22

    申请号：EP12856949.8

    申请日：2012-11-22

    申请人： Hitachi High-Technologies Corporation

    发明人： YAZAWA, Yoshiaki ,  YOKOI, Takahide ,  UEMATSU, Chihiro

    IPC分类号： C12N15/09 ,  C12M1/00 ,  C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6844 ,  C12Q2525/151 ,  C12Q2525/191 ,  C12Q2535/122

    摘要： An object of the present invention is to provide a method and means for amplifying a nucleic acid that reduce the time and effort required for amplification of template DNA and address the problems of conventional amplification methods, as well as a method and means for determining a nucleotide sequence using the same. The nucleic acid amplification method comprises the steps of: ligating a double-stranded adapter (20) containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA (1, 2) containing a target DNA sequence (1) to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (5); and performing a 3'-end elongation reaction using a strand-displacement DNA polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target DNA sequences (1) and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA, wherein the concatemer (29) contains a plurality of the target DNA sequences (1) suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.

    NUCLEIC ACID AMPLIFICATION METHOD
    4.
    发明公开
    NUCLEIC ACID AMPLIFICATION METHOD 审中-公开
    核酸扩增法

    公开(公告)号：EP2792743A1

    公开(公告)日：2014-10-22

    申请号：EP12856949.8

    申请日：2012-11-22

    申请人： Hitachi High-Technologies Corporation

    发明人： YAZAWA, Yoshiaki ,  YOKOI, Takahide ,  UEMATSU, Chihiro

    IPC分类号： C12N15/09 ,  C12M1/00 ,  C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6844 ,  C12Q2525/151 ,  C12Q2525/191 ,  C12Q2535/122

    摘要： An object of the present invention is to provide a method and means for amplifying a nucleic acid that reduce the time and effort required for amplification of template DNA and address the problems of conventional amplification methods, as well as a method and means for determining a nucleotide sequence using the same. The nucleic acid amplification method comprises the steps of: ligating a double-stranded adapter (20) containing adapter DNA strands capable of forming a folded structure to a double-stranded DNA (1, 2) containing a target DNA sequence (1) to prepare a cyclic DNA template composed of double-stranded DNA containing a nick (5); and performing a 3'-end elongation reaction using a strand-displacement DNA polymerase from the nick (5) as an origin, thereby producing a concatemer (29) in which a plurality of the target DNA sequences (1) and the adapter DNA strands capable of forming the folded structure are linked in series as a single-stranded DNA, wherein the concatemer (29) contains a plurality of the target DNA sequences (1) suitable for nucleotide sequence analysis and has a folded shape such that it takes the form of a ball due to its folded structure.

    摘要翻译： 本发明的一个目的是提供扩增核酸的方法和手段，该方法和手段减少扩增模板DNA所需的时间和精力，并解决常规扩增方法的问题，以及确定核苷酸的方法和手段 使用相同的序列。 核酸扩增方法包括以下步骤：将含有能够形成折叠结构的衔接头DNA链的双链衔接子（20）连接到含有目标DNA序列（1）的双链DNA（1,2）上以制备 由含有切口（5）的双链DNA组成的环状DNA模板; 使用来自切口（5）的链置换DNA聚合酶作为起点进行3'末端延伸反应，从而产生多个靶DNA序列（1）和接头DNA链的多联体（29） 能够形成折叠结构的单链DNA作为单链DNA串联连接，其中多联体（29）含有多个适于核苷酸序列分析的靶DNA序列（1），并具有折叠形状，使得其形式为 由于其折叠结构的球。

    BIOPOLYMER ANALYSIS METHOD, BIOPOLYMER ANALYZER, AND BIOPOLYMER ANALYSIS CHIP
    5.
    发明公开
    BIOPOLYMER ANALYSIS METHOD, BIOPOLYMER ANALYZER, AND BIOPOLYMER ANALYSIS CHIP 有权
    生物多样性分析仪

    公开(公告)号：EP2557158A1

    公开(公告)日：2013-02-13

    申请号：EP11762135.9

    申请日：2011-02-23

    申请人： Hitachi High-Technologies Corporation

    发明人： AKAHORI, Rena ,  ANAZAWA, Takashi ,  OZAWA, Satoshi ,  YAZAWA, Yoshiaki ,  SAKAI, Tomoyuki ,  NODA, Hideyuki

    IPC分类号： C12N15/09 ,  C12M1/00 ,  C12Q1/68 ,  G01N21/64 ,  G01N27/00 ,  G01N27/22 ,  G01N33/53 ,  G01N37/00 ,  G01N27/416

    CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  G01N33/48721 ,  C12Q2565/631

    摘要： There have been the following problems with sequence analysis using multiple nanopores: trapping a sample in the nanopores is not always 100% efficient and unnecessary time is spent to measure pores in which no sample has been trapped, resulting in low measurement efficiency. To address the problems, a labeling substance is boned to a sample, and the sample to which the labeling substance has been bonded is trapped in the nanopores. An apparatus for observing the labeling substance is used to observe the labeling substance and monitor whether or not the sample has been trapped in the nanopores. Measuring only nanopores in which the sample has been trapped allows the measurement efficiency to be improved.

    摘要翻译： 使用多个纳米孔的序列分析存在以下问题：在纳米孔中捕获样品并不总是100％的有效性，并且花费不需要的时间来测量没有样品被捕获的孔隙，导致低的测量效率。 为了解决这些问题，将标记物质结合到样品中，并且标记物质已经结合的样品被捕获在纳米孔中。 用于观察标记物质的装置用于观察标记物质并监测样品是否已被捕获在纳米孔中。 仅测量样品已被捕获的纳米孔，可以提高测量效率。