公开(公告)号：EP0949335A1

公开(公告)日：1999-10-13

申请号：EP97950371.1

申请日：1997-12-24

申请人： Hoechst Marion Roussel, Ltd.

发明人： HONDA, Jun, c/o Hoechst Marion Roussel Ltd. ,  ANDOU, Hidetoshi, c/o Hoechst Marion Roussel Ltd. ,  SUGIMOTO, Shunjiro, c/o Hoechst Marion Roussel Ltd

IPC分类号： C12P21/02 ,  C07K14/51 ,  C12N15/09

CPC分类号： C07K14/51

摘要： The present invention relates to a process for the production of purified dimeric bone morphogenetic factors. Purified dimeric bone morphogenetic factor can be produced by subjecting an inclusion body of a bone morphogenetic factor produced by means of a genetic engineering technology to the following steps a) - e) in order;

a) treating an inclusion body of a bone morphogenetic factor with a denaturing agent to obtain a solubilized monomer,
b) treating the solubilized monomer with a refolding solution to obtain a dimeric bone morphogenetic factor,
c) treating the dimeric bone morphogenetic factor by ultrafiltration and substitution of solvent,
d) subjecting the dimeric bone morphogenetic factor in solvent thus substituted to isoelectric precipitation, and
e) subjecting the dimeric bone morphogenetic factor thus isoelectrically precipitated to reverse-phase chromatography.

摘要翻译： 本发明涉及一种生产纯化的二聚体骨形态发生因子的方法。 纯化的二聚体骨形态发生因子可以通过使遗传工程技术产生的骨形态发生因子的包涵体按照以下步骤a）-e）进行制备： a）用变性剂处理骨形态发生因子的包涵体以获得溶解的单体，b）用重折叠溶液处理溶解的单体以获得二聚骨形态发生因子，c）通过超滤处理二聚体骨形态发生因子，和 取代溶剂，d）将二聚体骨形态发生因子置于溶剂中，从而代替等电点沉淀，e）使二聚体骨形态发生因子进行等电沉淀以进行反相色谱。