利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

7 条记录 (耗时 0.023 秒)

    REAGENTLESS FLUORESCENT BIOSENSORS COMPRISING A DESIGNED ANKYRIN REPEAT PROTEIN MODULE, RATIONAL DESIGN METHODS TO CREATE REAGENTLESS FLUORESCENT BIOSENSORS AND METHODS OF THEIR USE
    6.
    发明公开
    REAGENTLESS FLUORESCENT BIOSENSORS COMPRISING A DESIGNED ANKYRIN REPEAT PROTEIN MODULE, RATIONAL DESIGN METHODS TO CREATE REAGENTLESS FLUORESCENT BIOSENSORS AND METHODS OF THEIR USE 审中-公开
    WITH A锚蛋白重复蛋白模块基本原理施工工艺进行设计试剂批次荧光生物传感器用于生产REAGENZLOSER荧光生物传感器和使用方法

    公开(公告)号：EP2263088A2

    公开(公告)日：2010-12-22

    申请号：EP09723296.1

    申请日：2009-03-18

    申请人： Institut Pasteur ,  Centre National de la Recherche Scientifique

    发明人： BEDOUELLE, Hugues ,  BRIENT-LITZLER, Elodie

    IPC分类号： G01N33/542

    CPC分类号： G01N33/533 ,  G01N33/542

    摘要： The present invention relates to reagentless fluorescent biosensors which comprise at least one ankyrin repeat and a fluorophore and are specific for at least one target; the method for preparing such reagentless fluorescent biosensors comprises the following steps: (a) identifying the residues (R
    1 ) of the paratope of the biosensor by mutagenesis of all, or of a subset, of the residues of the biosensor, and determining variations in at least one measurable chemical or physical parameter of interaction with said at least one target; wherein said variations are due to each mutation or to groups of mutations; (b) selecting the cysteine residues, or the residues to be mutated to cysteine, from the residues (R
    2 ) of the biosensor which are located adjacent to the. residues of the paratope; (c) mutating by site-directed mutagenesis at least one of the residues (R
    2 ) selected in (b) to a cysteine residue when said residue is not naturally a cysteine residue; and (d) coupling the Sγ atom of at least one cysteine residue (R
    2 ) obtained in (b) or in (c) to a fluorophore.