公开(公告)号：EP3438278A1

公开(公告)日：2019-02-06

申请号：EP17775006.4

申请日：2017-03-28

申请人： Ikeda Food Research Co., Ltd. ,  Hiroshima City University

发明人： NAKATSUKA, Tomoko ,  AOKI, Hideyuki ,  KIDA, Mikiko ,  YAMADA, Kenta ,  KANEKO, Shoji ,  KUGIMIYA, Akimitsu

IPC分类号： C12Q1/25 ,  C12Q1/68 ,  G01N27/416 ,  G01N33/68

摘要： [Problem] To provide: a method for selectively and easily quantifying an amino acid to be measured (L-form or D-form amino acid) with high sensitivity using aminoacyl-tRNA synthetase (AARS); and an amino acid quantification kit. [Solution] The present invention pertains to: a method for quantifying an amino acid (L-form or D-form amino acid) in a sample by means of AARS, said method being characterized in that AARS and an amino acid are released from an aminoacyl-AMP AARS complex once formed and are used once again for forming an aminoacyl-AMP AARS complex so as to ultimately produce a reaction product such as pyrophoric acid to be measured in an amount that is greater in terms of the number of moles than the amino acid included in the sample; and an amino acid quantification kit for implementing said method.