SEQUENCING BY ORTHOGONAL SYNTHESIS
    2.
    发明授权
    SEQUENCING BY ORTHOGONAL SYNTHESIS 有权
    顺式DURCH ORTHOGONALE SYNTHESE

    公开(公告)号:EP3017063B1

    公开(公告)日:2017-04-05

    申请号:EP14739017.3

    申请日:2014-06-25

    申请人: Illumina, Inc.

    IPC分类号: C12Q1/68

    摘要: A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.

    摘要翻译: 一种用于测序的方法包括以下步骤:(a)提供第一和第二核酸模板,其中两个模板具有不同的序列; (b)使用第一聚合酶种类和第一组核苷酸类似物扩展与第一模板结合的第一引物; (c)使用第二聚合酶种类和第二组核苷酸类似物扩展与第二模板结合的第二引物,其中第一聚合酶种类与第二聚合酶种类不同,并且其中第一组核苷酸类似物不同于第二种 (d)检测第一和第二引物延伸产物; 和(e)重复步骤(b)至(d),从而确定第一和第二模板的不同序列。

    SYSTEM FOR SEQUENCING BY ORTHOGONAL SYNTHESIS
    3.
    发明公开
    SYSTEM FOR SEQUENCING BY ORTHOGONAL SYNTHESIS 审中-公开
    正交合成法测序系统

    公开(公告)号:EP3241913A1

    公开(公告)日:2017-11-08

    申请号:EP17164818.1

    申请日:2014-06-25

    申请人: Illumina, Inc.

    IPC分类号: C12Q1/68

    摘要: A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.

    摘要翻译: 测序方法包括以下步骤:(a)提供第一和第二核酸模板,其中两个模板具有不同的序列; (b)使用第一聚合酶物种和第一组核苷酸类似物延伸与第一模板结合的第一引物; (c)使用第二聚合酶物种和第二组核苷酸类似物延伸与第二模板结合的第二引物,其中第一聚合酶物种不同于第二聚合酶物种,并且其中第一组核苷酸类似物不同于第二种 核苷酸类似物组,(d)检测第一和第二引物延伸产物; 和(e)重复步骤(b)到(d),由此确定第一和第二模板的不同序列。