公开(公告)号：EP3698874A1

公开(公告)日：2020-08-26

申请号：EP20165370.6

申请日：2015-03-11

申请人： Illumina, Inc.

发明人： SABOUNCHI, Poorya ,  JAVANMARDI, Behnam ,  KHURANA, Tarun ,  PAIK, Philip ,  LIN, Yan-You

IPC分类号： B01L3/00 ,  C12Q1/6806 ,  B01L7/00 ,  C12Q1/6869

摘要： The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

公开(公告)号：EP3116651B1

公开(公告)日：2020-04-22

申请号：EP15711627.8

申请日：2015-03-11

申请人： Illumina, Inc.

发明人： SABOUNCHI, Poorya ,  JAVANMARDI, Behnam ,  KHURANA, Tarun ,  PAIK, Philip ,  LIN, Yan-You

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/6806 ,  C12Q1/6869

公开(公告)号：EP3912724A1

公开(公告)日：2021-11-24

申请号：EP21180636.9

申请日：2019-05-01

申请人： Illumina, Inc.

发明人： DELATTRE, Cyril ,  RHEE, Minsoung ,  LIU, Jeffrey ,  COX-MURANAMI, Wesley ,  CRIVELLI, Paul ,  FOLEY, Jennifer ,  SEGALE, Darren ,  TAYLOR, Jay ,  HAGE, Matthew ,  PAIK, Philip ,  ALLEGOREN, Erik ,  HERTZOG, David ,  MOROZ-SMIETANA, Alex ,  MA, Xiaoxiao ,  TAKAHASHI, Tsukasa ,  WESTERBERG, Brandon

IPC分类号： B01L3/00 ,  F16L11/22

摘要： An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

公开(公告)号：EP3628025A1

公开(公告)日：2020-04-01

申请号：EP19802686.6

申请日：2019-05-01

申请人： Illumina Inc.

发明人： DELATTRE, Cyril ,  RHEE, Minsoung ,  LIU, Jeffrey ,  COX-MURANAMI, Wesley ,  CRIVELLI, Paul ,  FOLEY, Jennifer ,  SEGALE, Darren ,  TAYLOR, Jay ,  HAGE, Matthew ,  PAIK, Philip ,  ALLEGOREN, Erik ,  HERTZOG, David ,  MOROZ-SMIETANA, Alex ,  MA, Xiaoxiao ,  TAKAHASHI, Tsukasa ,  WESTERBERG, Brandon

IPC分类号： B01L3/00 ,  F16L11/22

公开(公告)号：EP3116651A1

公开(公告)日：2017-01-18

申请号：EP15711627.8

申请日：2015-03-11

申请人： Illumina, Inc.

发明人： SABOUNCHI, Poorya ,  JAVANMARDI, Behnam ,  KHURANA, Tarun ,  PAIK, Philip ,  LIN, Yan-You

IPC分类号： B01L7/00 ,  B01L3/00

摘要： The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

摘要翻译： 盘游离缺失实施例涉及用于检测生物反应的微流体盒。 在一些实施方案中，微流体盒被配置为对核酸样品进行测序操作。 在一个方面，微流体盒包括堆叠层流体限定通道和阀，用于处理核酸样品待测序的，以及固态CMOS生物传感器集成在所述堆栈中。 该生物传感器具有被配置为检测生物反应的信号的有效面积，基本上所有worin有源区的可用于在操作期间试剂递送和照明。 在另一个方面中，微流体盒包括：（a）一种流动池包含反应位点区域包含一个或多个反应位点; （B）流体通道用于输送反应物和/或除去反应位点区的反应物; （C）具有在被配置为检测在反应位点区域的生物反应的信号有效区域的生物传感器。 反应位点区域是靠近所述生物传感器和所述反应位点区的有源区基本上跨越所有生物传感器的活性区域。 在一些实施例中，流体通道基本上不与生物传感器的活性区域重叠。 制造和操作微流体卡盒的方法是使游离缺失盘。