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    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES
    1.
    发明公开
    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 有权
    方法多核苷酸的选择和扩增

    公开(公告)号：EP2470669A1

    公开(公告)日：2012-07-04

    申请号：EP09848830.7

    申请日：2009-08-25

    申请人： Illumina, Inc.

    发明人： SABOT, Andrea ,  RIGATTI, Roberto ,  SHEN, Min-jui, Richard

    IPC分类号： C12P19/34

    CPC分类号： C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/507 ,  C12Q2531/107

    摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES
    3.
    发明公开
    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 审中-公开
    VERFAHREN ZUR AUSWAHL UND AMPLIFATIONATION VON POLYNUKLEOTIDEN

    公开(公告)号：EP3133169A1

    公开(公告)日：2017-02-22

    申请号：EP16180028.9

    申请日：2009-08-25

    申请人： Illumina, Inc.

    发明人： SABOT, Andrea ,  RIGATTI, Roberto ,  SHEN, Min-jui, Richard

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/507 ,  C12Q2531/107

    摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

    摘要翻译： 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到与固体支持物连接的不同捕获分子。 可以可逆地修饰一个或多个捕获分子，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES
    4.
    发明授权
    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 有权
    方法多核苷酸的选择和扩增

    公开(公告)号：EP2470669B1

    公开(公告)日：2014-06-18

    申请号：EP09848830.7

    申请日：2009-08-25

    申请人： Illumina, Inc.

    发明人： SABOT, Andrea ,  RIGATTI, Roberto ,  SHEN, Min-jui, Richard

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/507 ,  C12Q2531/107

    摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.