公开(公告)号：EP4190889A1

公开(公告)日：2023-06-07

申请号：EP22207633.3

申请日：2014-08-07

申请人： Illumina, Inc.

发明人： Stone, Michael ,  Verkade, Drew

IPC分类号： C12M1/38 ,  C12Q1/68 ,  G01N35/10

摘要： The invention pertains to a fluidic system configured to allow re-use of one or more reagents, the system comprising a reagent reservoir; a flow cell; a manifold configured such that the reagent reservoir is in fluid communication with the flow cell through one or more lines; wherein a portion of the line comprises a cache reservoir configured to receive liquid reagent from the reagent reservoir, and configured to provide a volume of the liquid reagent into a line of the flow cell, and configured to receive at least a portion of the liquid reagent back from the flow cell for re-use in the flow cell and maintain a gradient of the portion of the liquid reagent back from the flow cell and fresh liquid reagent within the cache reservoir; and wherein the cache reservoir is in fluid communication with a pump configured to move liquid reagent from the cache reservoir to the flow cell and from the flow cell back to the cache reservoir. The invention also pertains to a method of reagent re-use.