公开(公告)号：EP3149198A1

公开(公告)日：2017-04-05

申请号：EP15728090.0

申请日：2015-06-02

申请人： Illumina Cambridge Limited

发明人： BOUTELL, Jonathan Mark ,  SHANAHAN, Susan ,  RIGATTI, Roberto

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6846 ,  C12Q1/6806 ,  C12Q2523/113 ,  C12Q2527/125 ,  C12Q2565/543 ,  C12Q2527/101 ,  C12Q2531/119 ,  C12Q2535/122

摘要： The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

摘要翻译： 本发明提供了用于扩增核酸的方法，特别是用于降低密度依赖性GC偏差的方法和用于降低核酸模板的桥连扩增中的核酸损伤。 本发明还提供了用于评估试剂和/或添加剂对核酸模板链桥联扩增期间的核酸损伤的影响的方法。 该方法适用于固相扩增，例如利用流动池。

公开(公告)号：EP3149198B1

公开(公告)日：2018-10-24

申请号：EP15728090.0

申请日：2015-06-02

申请人： Illumina Cambridge Limited

发明人： BOUTELL, Jonathan Mark ,  SHANAHAN, Susan ,  RIGATTI, Roberto

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6846 ,  C12Q1/6806 ,  C12Q2523/113 ,  C12Q2527/125 ,  C12Q2565/543 ,  C12Q2527/101 ,  C12Q2531/119 ,  C12Q2535/122

摘要： The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.