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    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION
    1.
    发明公开
    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION 审中-公开
    组合物和方法从复杂的混合物杂交NUCLEIC代表选择

    公开(公告)号：EP2076609A1

    公开(公告)日：2009-07-08

    申请号：EP07837278.6

    申请日：2007-08-24

    申请人： Illumina Inc.

    发明人： STUELPNAGEL, John R. ,  BARKER, David L. ,  VELARDE, Jorge Jr. ,  BARNARD, Steven M. ,  GRAIGE, Michael

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6837 ,  C12Q2565/515

    摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

    MULTI-PLANE MICROARRAYS
    3.
    发明公开
    MULTI-PLANE MICROARRAYS 审中-公开

    公开(公告)号：EP3831484A1

    公开(公告)日：2021-06-09

    申请号：EP20217230.0

    申请日：2017-03-28

    申请人： Illumina, Inc.

    发明人： BOWEN, Shane ,  GRAIGE, Michael ,  HONG, Stanley ,  MOON, John ,  SIU, Merek

    IPC分类号： B01L3/00 ,  B01J19/00 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6874

    摘要： An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z 1 and the features of the second pattern occur at a second elevation z 2 . The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.