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    SELECTION OF CELLS EXPRESSING HETEROMERIC POLYPEPTIDES
    1.
    发明授权
    SELECTION OF CELLS EXPRESSING HETEROMERIC POLYPEPTIDES 有权

    公开(公告)号：EP3187592B1

    公开(公告)日：2018-12-12

    申请号：EP16200934.4

    申请日：2002-09-20

    申请人： Immunex Corporation

    发明人： MCGREW, Jeffrey, T. ,  BIANCHI, Allison, A.

    IPC分类号： C12P21/08 ,  C12Q1/68 ,  C07K16/28 ,  C12N15/62

    CPC分类号： C07K16/2866 ,  C07K2319/00 ,  C07K2319/73 ,  C12N15/62

    摘要： The present invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More particularly, the invention concerns improved selection in cells of recombinantly engineered vectors designed to express polypeptides. It relates more specifically to a method of producing and isolating a heteromeric immunoglobulin heavy and light chain complex, the method comprising transfecting cells with a vector comprising a first nucleic acid encoding a first immunoglobulin heavy or light chain, wherein the transcription of said first nucleic acid is operably linked to transcription of a second nucleic acid encoding a first subunit of a selectable marker, and further comprising a third nucleic acid encoding a second immunoglobulin light or heavy chain wherein the immunoglobulin light and heavy chains are capable of associating to form a heteromeric complex, wherein the transcription of said third nucleic acid is operably linked to transcription of a fourth nucleic acid which encodes a second subunit of a selectable marker, and wherein said second and first subunit of the selectable marker associate, to provide a selectable activity, and applying selection to the cells.

    SELECTION OF CELLS EXPRESSING HETEROMERIC POLYPEPTIDES
    3.
    发明公开
    SELECTION OF CELLS EXPRESSING HETEROMERIC POLYPEPTIDES 审中-公开
    表达异源多肽的细胞的选择

    公开(公告)号：EP3187592A1

    公开(公告)日：2017-07-05

    申请号：EP16200934.4

    申请日：2002-09-20

    申请人： Immunex Corporation

    发明人： MCGREW, Jeffrey, T. ,  BIANCHI, Allison, A.

    IPC分类号： C12P21/08 ,  C12Q1/68 ,  C07K16/28 ,  C12N15/62

    CPC分类号： C07K16/2866 ,  C07K2319/00 ,  C07K2319/73 ,  C12N15/62

    摘要： The present invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More particularly, the invention concerns improved selection in cells of recombinantly engineered vectors designed to express polypeptides. It relates more specifically to a method of producing and isolating a heteromeric immunoglobulin heavy and light chain complex, the method comprising transfecting cells with a vector comprising a first nucleic acid encoding a first immunoglobulin heavy or light chain, wherein the transcription of said first nucleic acid is operably linked to transcription of a second nucleic acid encoding a first subunit of a selectable marker, and further comprising a third nucleic acid encoding a second immunoglobulin light or heavy chain wherein the immunoglobulin light and heavy chains are capable of associating to form a heteromeric complex, wherein the transcription of said third nucleic acid is operably linked to transcription of a fourth nucleic acid which encodes a second subunit of a selectable marker, and wherein said second and first subunit of the selectable marker associate, to provide a selectable activity, and applying selection to the cells.

    摘要翻译： 本发明涉及动物细胞培养物中多肽重组表达的一般领域。 更具体地说，本发明涉及改进的设计用于表达多肽的重组工程载体在细胞中的选择。 本发明更具体地涉及生产和分离异源免疫球蛋白重链和轻链复合物的方法，所述方法包括用包含编码第一免疫球蛋白重链或轻链的第一核酸的载体转染细胞，其中所述第一核酸 与编码选择性标记的第一亚基的第二核酸的转录可操作地连接，并且还包含编码第二免疫球蛋白轻链或重链的第三核酸，其中所述免疫球蛋白轻链和重链能够缔合形成异聚复合物 其中所述第三核酸的转录与编码选择性标记的第二亚基的第四核酸的转录可操作地连接，并且其中所述选择性标记的所述第二和第一亚基缔合以提供可选活性， 选择到细胞。