公开(公告)号：EP3912723A1

公开(公告)日：2021-11-24

申请号：EP21170608.0

申请日：2016-03-22

申请人： InSilixa, Inc.

发明人： HASSIBI, Arjang ,  JIRAGE, Kshama ,  MANICKAM, Arun ,  MILANINIA, Kaveh

IPC分类号： B01L3/00 ,  C12Q1/68 ,  C40B30/04 ,  G01N33/48 ,  H01L21/302

摘要： The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function of temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.

公开(公告)号：EP3274094A1

公开(公告)日：2018-01-31

申请号：EP16769558.4

申请日：2016-03-22

申请人： InSilixa Inc.

发明人： HASSIBI, Arjang ,  JIRAGE, Kshama ,  MANICKAM,Arun ,  MILANINIA, Kaveh

IPC分类号： B01L3/00 ,  C12Q1/68 ,  C40B30/04 ,  G01N33/48 ,  H01L21/302

CPC分类号： C12Q1/6837 ,  C12Q2527/107 ,  C12Q2565/101 ,  C12Q2565/607

摘要： The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.