公开(公告)号：EP3237602A1

公开(公告)日：2017-11-01

申请号：EP15830866.8

申请日：2015-12-23

申请人： Institut Pasteur ,  Fondation Imagine ,  Assistance Publique-Hôpitaux de Paris ,  Université Paris Descartes ,  Institut National de la Santé et de la Recherche Médicale (INSERM)

发明人： EBERL, Gérard ,  BIKARD, David ,  SCHNUPF, Pamela ,  CERF BENSUSSAN, Nadine ,  GABORIAU-ROUTHIAU, Valérie ,  SANSONETTI, Philippe

IPC分类号： C12N1/20

CPC分类号： C12N1/20 ,  C12N13/00 ,  C12N15/74 ,  C12N2502/23

摘要： The present invention relates to an in vitro method of culturing a segmented filamentous bacterium strain, comprising co-culturing said segmented filamentous bacterium strain with a eukaryotic host cell, wherein the culture is performed at an O2 level inferior to 5% in a rich tissue culture liquid medium containing bacterial medium components including iron. The present invention also relates to methods for genetically modifying a segmented filamentous bacterium strain comprising a step a culturing the strain in vitro.

摘要翻译： 本发明涉及培养分段丝状细菌菌株的体外方法，其包括将所述分段丝状细菌菌株与真核宿主细胞共培养，其中所述培养物在富含组织中以低于5％的O 2水平进行 含有含铁细菌培养基成分的培养液培养基。 本发明还涉及用于遗传修饰分段丝状细菌菌株的方法，其包括在体外培养菌株的步骤。