公开(公告)号：EP3265586A1

公开(公告)日：2018-01-10

申请号：EP16732333.6

申请日：2016-03-04

申请人： Institut Pasteur ,  Université Paris Diderot - Paris 7

发明人： WEIL, Robert ,  MESSALI, Nassima ,  SOARES, Helena ,  NAOKO, Satoh-Takayama ,  TRIVELLAS, Andromahi ,  DI SANTO, James ,  ALCOVER, Andrés

IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/574

CPC分类号： C12Q1/6886 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2217/077 ,  A01K2227/105 ,  A01K2267/0387 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/156 ,  G01N33/57407 ,  G01N2500/10

摘要： The invention provides methods of identifying an E-SYT2 modulator. The methods may comprise providing a cell that expresses E-SYT2; contacting the cell with a candidate chemical entity; and characterizing recruitment of at least one of Carma1, BcllO, NEMO, and PKC9 to the immunological synapse (IS). The invention also provides E-SYT2 modulators, including inhibitors, identified using the disclosed methods. The invention provides methods of inhibiting NF-κB activity in a cell comprising contacting the cell with an E-SYT2 inhibitor. The invention provides methods comprising providing a sample from a patient suspected of having or at risk of having a MALT-lymphoma or an ABC-DLBCL-lymphoma; and screening the sample to identify the presence and/or absence of a gain of function E-Syt2 mutation in the sample. The invention also provides genetically modified mammals comprising one or two loss of function alleles of E-Syt2.