公开(公告)号：EP2395088A1

公开(公告)日：2011-12-14

申请号：EP10179712.4

申请日：2010-09-24

申请人： Instytut Biochemii I Biofizyki Pan

发明人： Liszewska, Frantz ,  Wawrzynska, Anna ,  Sirko, Agnieszka

IPC分类号： C12N15/82 ,  C12N9/10

CPC分类号： C12N15/8253 ,  C12N9/1085

摘要： The present invention provides the means and the methods for obtaining plant materials producing large amounts of functionally active cysteine synthase (EC 2.5.1.47) that rely on an environment-friendly and health-safe enzyme production process for synthesis of L-cysteine and L-cystine, therefore suitably accounting among the c-GMP standards for production of the aforementioned compounds, namely of non-human and non-animal origin. Described is a recombinant DNA molecule comprising a nucleic acid molecule encoding a protein having cysteine synthase activity, wherein the said nucleic acid molecule is operably linked to regulatory elements allowing the expression of the nucleic acid molecule in plant cells. The present invention also provides a vector harboring the plant expression cassette comprising the said recombinant DNA molecule, as well as plant cells, plant tissues and plants transformed therewith. The present invention further describes the use of the aforementioned recombinant DNA molecule and vector in plant cell culture, plant tissue culture, and plant breeding; further presented are examples of the use of the said plant cells, plant tissues, plants, as well as the extracts from these plant raw materials for biosynthetic production of L-cysteine and/or L-cystine compounds being valuables in a broad range of applications, such as for production of additives or ingredients used in agroindustrial, cosmetic and pharmaceutic processes.

摘要翻译： 本发明提供了一种方式，并获得规划教材生产功能性活性半胱氨酸合酶的大量方法（EC 2.5.1.47）做依靠，为L-半胱氨酸的合成环境友好和健康安全的酶制剂生产过程L- 胱氨酸，故适当的c GMP标准中占生产上述化合物的，即非人类和非动物来源。 描述了包含编码具有蛋白半胱氨酸合酶活性的核酸分子，所述worin所述核酸分子可操作地连接至允许在植物细胞中的核酸分子的表达的调控元件的重组DNA分子。 因此，本发明提供了一个载体携带植物表达盒包含所述重组DNA分子，以及规划细胞，植物组织，并用转化的有植物。 本发明还描述了使用在植物细胞培养物，植物组织培养物，和植物育种前述重组DNA分子和载体; 进一步呈现是利用所述植物细胞，植物组织，植物，以及从合成提取物规划原料生物合成生产L-半胱氨酸和/或L-胱氨酸化合物是贵重物品在宽范围的应用中的示例 ，：如用于生产农工业，化妆品和药用过程中使用的添加剂或成分。