公开(公告)号：EP2757373A1

公开(公告)日：2014-07-23

申请号：EP12832236.9

申请日：2012-09-13

申请人： International Institute of Cancer Immunology, Inc. ,  Otsuka Pharmaceutical Co., Ltd.

发明人： SUGIYAMA, Haruo ,  OJI, Yusuke ,  KATSURAGI, Kiyonori ,  TANAKA, Hideaki ,  SOGO, Shinji ,  GOTO, Yoshihiro ,  OHMOTO, Yasukazu ,  IWATA, Husako

IPC分类号： G01N33/53 ,  C07K14/82 ,  C12N15/09 ,  A61K39/00 ,  G01N33/574

CPC分类号： G01N33/574 ,  A61K39/0011 ,  C07K14/82 ,  G01N33/57407 ,  G01N33/57419 ,  G01N33/57426 ,  G01N33/57488 ,  G01N33/6854

摘要： Provided is an invention relating to a method for measuring an anti-WT1 antibody in a sample allowing measurement and evaluation of the anti-WT1 antibody with higher accuracy and to use of the method. The method for measuring an anti-WT1 antibody in a sample includes using a polypeptide having antigenicity to the anti-WT1 antibody selected from a polypeptide comprising the amino acid sequence of positions 294-449 in SEQ ID NO: 1, a partial polypeptide of the polypeptide, and a polypeptide including an amino acid sequence having deletion, substitution, or addition of one to several amino acids in the amino acid sequence constituting each of these polypeptides and/or a polypeptide having antigenicity to the anti-WT1 antibody selected from a polypeptide comprising the amino acid sequence of positions 181-324 in SEQ ID NO: 1, a partial polypeptide of the polypeptide, and a polypeptide including an amino acid sequence having deletion, substitution, or addition of one to several amino acids in the amino acid sequence constituting each of these polypeptides.

摘要翻译： 本发明涉及一种测定样品中的抗WT1抗体的方法的发明，其能够更准确地测定和评价抗WT1抗体并使用该方法。 用于测量样品中抗WT1抗体的方法包括使用对抗WT1抗体具有抗原性的多肽，所述抗WT1抗体选自包含SEQ ID NO：1中294-449位氨基酸序列的多肽， 多肽和包含在构成每个这些多肽的氨基酸序列中具有1至数个氨基酸的缺失，取代或添加的氨基酸序列的多肽和/或对抗WT1抗体具有抗原性的多肽，所述抗WT1抗体选自多肽 其包含SEQ ID NO：1中的位置181-324的氨基酸序列，多肽的部分多肽，以及包含在氨基酸序列中具有一至几个氨基酸的缺失，取代或添加的氨基酸序列的多肽 构成这些多肽中的每一种。