公开(公告)号：EP1597584A1

公开(公告)日：2005-11-23

申请号：EP04713111.5

申请日：2004-02-20

申请人： Ipsen Limited

发明人： PICKETT, Andrew Martin,Ipsen Limited ,  QUIRK, Robin Andrew,Regence Limited ,  FRANCE, Richard Melville,Regentec Limited ,  RICCALTON-BANKS, Lisa Anne,Regentec Limited

IPC分类号： G01N33/68 ,  G01N33/94 ,  G01N33/50

CPC分类号： G01N33/944 ,  G01N33/5088

摘要： The invention relates to a method for determining the quantity of pre-synaptic neuromuscular blocking substance (notably botulinum toxin) contained in a sample. In one aspects, the method comprises the following steps: (i) determining the minimum voltage Vm needed to induce the contraction of muscle tissue, said muscle tissue being connected to an electrical stimulator through a motor nerve and preferably immersed in an oxygenated physiological buffer containing glucose; (ii) adding the sample containing the pre-synaptic neuromuscular blocking substance; (iii) electrically stimulating, at a voltage at least equal to Vm, the muscle tissue at certain time intervals; (iv) comparing the effect induced by the sample to the effect induced by a reference substance and thereby determining the quantity of the pre-synaptic neuromuscular blocking substance in the sample.