公开(公告)号：EP1809753A1

公开(公告)日：2007-07-25

申请号：EP05801950.6

申请日：2005-10-27

申请人： Istituto di Richerche di Biologia Molecolare P. Angeletti S.p.A.

发明人： CATALUCCI, Daniele ,  COLLOCA, Stefano

IPC分类号： C12N15/861

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2800/108 ,  C12N2820/60 ,  C12N2830/006

摘要： The present invention relates to a plasmid that can be used for the development of efficient producer cell lines for the production of helper independent adenovirus vectors carrying multiple deletions of non-structural as well as structural genes. More specifically, the present invention provides producer cells which comprise a novel adenoviral amplicon that can be used to complement a multi-deleted adenoviral vectors and obtain high titer preparations. The amplicon is an episomal plasmid that expresses Ad5 E2 viral genes (i.e.,polymerase, pre-terminal protein and DNA binding protein) and E4 orf6 , the EBV the latent origin of replication (OriP) as well as adenoviral origins of replications in form of a covalent junction of left and right ITRs. This plasmid is capable of self-replication upon induction of Ad5 E2 gene expression. The invention further includes methods for the preparation of the disclosed producer cells and uses of the cells to produce viral vectors on a scale that is sufficient for therapeutic uses.